CAJ | 학술논문

目的：探讨1，25-二羟维生素D3[1，25（OH）2D3]对人系膜细胞增殖与凋亡的影响。方法：体外培养人系膜细胞，随机分为4组：正常对照组、表皮生长因子（EGF）（10 ng/mL）组、1，25（OH）2D3（10-8 mol/L）组、EGF 联合1，25（OH）2D3干预组，采用CCK-8法检测各组干预48 h 后系膜细胞的增殖情况，流式细胞术检测各组干预48 h 后系膜细胞周期分布情况， TUNEL 法检测各组干预48 h 后系膜细胞凋亡情况。结果：与正常对照组相比，EGF组能显著促进系膜细胞增殖，G1期细胞显著减少，S期细胞显著增加，且凋亡指数显著降低（P＜0．01），1，25（OH）2D3组系膜细胞增殖显著受抑制，G1期细胞增加，S期细胞减少，且凋亡指数增高（P＜0．01）；与EGF组比较，EGF联合1，25（OH）2D3干预组系膜细胞增殖显著受抑制，G1期细胞显著增多， S期细胞显著减少，且凋亡指数显著增高（P＜0．01或P＜0．05）。结论：1，25（OH）2D3可显著抑制人系膜细胞的增殖，促进其凋亡，且1，25（OH）2D3可抑制EGF对系膜细胞的促增殖作用及抑制凋亡的作用。
목적：탐토1，25-이간유생소D3[1，25（OH）2D3]대인계막세포증식여조망적영향。방법：체외배양인계막세포，수궤분위4조：정상대조조、표피생장인자（EGF）（10 ng/mL）조、1，25（OH）2D3（10-8 mol/L）조、EGF 연합1，25（OH）2D3간예조，채용CCK-8법검측각조간예48 h 후계막세포적증식정황，류식세포술검측각조간예48 h 후계막세포주기분포정황， TUNEL 법검측각조간예48 h 후계막세포조망정황。결과：여정상대조조상비，EGF조능현저촉진계막세포증식，G1기세포현저감소，S기세포현저증가，차조망지수현저강저（P＜0．01），1，25（OH）2D3조계막세포증식현저수억제，G1기세포증가，S기세포감소，차조망지수증고（P＜0．01）；여EGF조비교，EGF연합1，25（OH）2D3간예조계막세포증식현저수억제，G1기세포현저증다， S기세포현저감소，차조망지수현저증고（P＜0．01혹P＜0．05）。결론：1，25（OH）2D3가현저억제인계막세포적증식，촉진기조망，차1，25（OH）2D3가억제EGF대계막세포적촉증식작용급억제조망적작용。
Objective To investigate the effects of 1,25-dihydroxyvitamin D3 on the proliferation and apoptosis of cultured human glomerular mesangial cells. Methods The cultured human glomerular mesangial cells were divided into four groups: the normal control group, the EGF (10 ng/mL) group, the 1,25-dihydroxyvitamin D3 (10-8 mol/L) group and the EGF in combination with 1,25-dihydroxyvitamin D3 group. Afterr 48-hours treatment, cell proliferation was measured by cell counting kit-8 (cck-8) assay, cell cycle was measured by flow cytometry, and cell apoptosis was measured by the TdT-mediated dUTP nick-end labeling (TUNEL) assay. Results Compared with the normal control, EGF could significantly promote the proliferation of human glomerular mesangial cells (P<0.01). The percentage of cells with G1 phase significantly decreased and the percentage of cells with S phase significantly increased (P < 0.01). The apoptosis index of the human glomerular mesangial cells significantly decreased in the EGF group (P < 0.01). 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation of human glomerular mesangial cells (P<0.01). 1,25-dihydroxyvitamin D3 could significantly increase the percentage of G1 phase cells and could decrease the percentage of S phase cells (P<0.01), and could significantly increase the apoptosis index of human glomerular mesangial cells (P<0.01). Compared with EGF, EGF in combination with 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation of human glomerular mesangial cells (P <0.01). The percentage of G1 phase cells significantly increased and the percentage of S phase cellssignificantly decreased (P < 0.01), and the cell apoptosis index significantly increased in the EGF in combination with 1,25-dihydroxyvitamin D3 group (P < 0.05). Conclusions (1) 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation and promote the apoptosis of human glomerular mesangial cells. (2) 1,25-dihydroxyvitamin D3 could also inhibit the proliferation of EGF-induced human glomerular mesangial cells.