CAJ | 학술논문

通过吸附和解吸附实验,从6种大孔树脂中筛选出适于分离纯化废次烟叶中烟草多酚的大孔树脂,并确定该大孔树脂纯化工艺参数。结果显示,D101大孔树脂能够较好的富集纯化废次烟叶中的烟草多酚,当提取液浓度为2.5 mg/mL,pH值为4.0,上样液流速为2.0 mL/min,洗脱液为80%乙醇溶液,洗脱液流速为2.0 mL/min时,纯化效果最好,烟草多酚的动态回收率为70.37%。
통과흡부화해흡부실험,종6충대공수지중사선출괄우분리순화폐차연협중연초다분적대공수지,병학정해대공수지순화공예삼수。결과현시,D101대공수지능구교호적부집순화폐차연협중적연초다분,당제취액농도위2.5 mg/mL,pH치위4.0,상양액류속위2.0 mL/min,세탈액위80%을순용액,세탈액류속위2.0 mL/min시,순화효과최호,연초다분적동태회수솔위70.37%。
Absorption and desorption experiments were performed to screen the suitable resin from 6 types of macroporous absorbent resins for the purification of tobacco polyphenol from discarded tobacco leaves.Results indicate that macroporous resin D101 possesses the strongest absorption and desorption capacity.The optimal absorption and desorption condition of macroporous resin D101 is the concentration of feeding solution 2.5 mg/mL,pH value 4.0,feeding rate 2.0 mL/min,elute ethanol concentration 80%,elution rate 2.0 mL/min.The dynamic recovery of tobacco polyphenol was 70.37% under the optimal condition.