中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
11期
1615-1617,后插3
,共4页
张红星%龚燕萍%许国华%管梅影%金一%赵向红%蒋廷旺%徐坚
張紅星%龔燕萍%許國華%管梅影%金一%趙嚮紅%蔣廷旺%徐堅
장홍성%공연평%허국화%관매영%금일%조향홍%장정왕%서견
肝炎,乙型,慢性%B淋巴细胞%白细胞介素10
肝炎,乙型,慢性%B淋巴細胞%白細胞介素10
간염,을형,만성%B림파세포%백세포개소10
Hepatitis,type B,chronic%B-lymphocytes%Interleukin-10
目的 分析慢性乙型病毒性肝炎(简称慢性乙肝,CHB)患者外周血IL-10+ CD19+调节性B细胞(Breg)的表达情况及其临床意义.方法 选取CHB患者61例,其中HBeAg阳性患者26例,对照组30例,抽取EDTA抗凝外周血,分离外周血单个核细胞(PBMCs),anti-IL-10-PE,anti-CD19-APC抗体进行染色后,流式细胞术分析IL-10+ CD19+ Breg的表达情况,同时分析HBeAg阳性和阴性患者Breg表达差异以及Breg与CHB临床指标的相关性;ELISA分析血清IL-10表达水平.结果 CHB和HCs组Breg分别为1.75％(1.20％ ～2.55％)和0.78％(0.55％～1.40％),CHB组Breg表达高于HCs组,两组差异有统计学意义(Z=-4.54,P＜0.01);CHB患者中HBeAg阳性患者Breg为2.39％(1.68％～2.86％),较阴性患者1.28％(1.05％～2.25％)显著升高(Z=-3.354,P=0.001);CHB和HCs组血清IL-10分别为6.80(5.41～8.42) pg/mL和5.75 (4.40～6.54) pg/mL,两者差异有统计学意义(Z=-2.697,P=0.007),并且CHB组IL-10与Breg呈正相关关系(r =0.335,P=0.008).结论 慢性乙肝患者外周血Breg表达显著升高,且可能受到HBeAg的影响.
目的 分析慢性乙型病毒性肝炎(簡稱慢性乙肝,CHB)患者外週血IL-10+ CD19+調節性B細胞(Breg)的錶達情況及其臨床意義.方法 選取CHB患者61例,其中HBeAg暘性患者26例,對照組30例,抽取EDTA抗凝外週血,分離外週血單箇覈細胞(PBMCs),anti-IL-10-PE,anti-CD19-APC抗體進行染色後,流式細胞術分析IL-10+ CD19+ Breg的錶達情況,同時分析HBeAg暘性和陰性患者Breg錶達差異以及Breg與CHB臨床指標的相關性;ELISA分析血清IL-10錶達水平.結果 CHB和HCs組Breg分彆為1.75％(1.20％ ～2.55％)和0.78％(0.55％～1.40％),CHB組Breg錶達高于HCs組,兩組差異有統計學意義(Z=-4.54,P＜0.01);CHB患者中HBeAg暘性患者Breg為2.39％(1.68％～2.86％),較陰性患者1.28％(1.05％～2.25％)顯著升高(Z=-3.354,P=0.001);CHB和HCs組血清IL-10分彆為6.80(5.41～8.42) pg/mL和5.75 (4.40～6.54) pg/mL,兩者差異有統計學意義(Z=-2.697,P=0.007),併且CHB組IL-10與Breg呈正相關關繫(r =0.335,P=0.008).結論 慢性乙肝患者外週血Breg錶達顯著升高,且可能受到HBeAg的影響.
목적 분석만성을형병독성간염(간칭만성을간,CHB)환자외주혈IL-10+ CD19+조절성B세포(Breg)적표체정황급기림상의의.방법 선취CHB환자61례,기중HBeAg양성환자26례,대조조30례,추취EDTA항응외주혈,분리외주혈단개핵세포(PBMCs),anti-IL-10-PE,anti-CD19-APC항체진행염색후,류식세포술분석IL-10+ CD19+ Breg적표체정황,동시분석HBeAg양성화음성환자Breg표체차이이급Breg여CHB림상지표적상관성;ELISA분석혈청IL-10표체수평.결과 CHB화HCs조Breg분별위1.75％(1.20％ ～2.55％)화0.78％(0.55％～1.40％),CHB조Breg표체고우HCs조,량조차이유통계학의의(Z=-4.54,P＜0.01);CHB환자중HBeAg양성환자Breg위2.39％(1.68％～2.86％),교음성환자1.28％(1.05％～2.25％)현저승고(Z=-3.354,P=0.001);CHB화HCs조혈청IL-10분별위6.80(5.41～8.42) pg/mL화5.75 (4.40～6.54) pg/mL,량자차이유통계학의의(Z=-2.697,P=0.007),병차CHB조IL-10여Breg정정상관관계(r =0.335,P=0.008).결론 만성을간환자외주혈Breg표체현저승고,차가능수도HBeAg적영향.
Objective To investigate the expression of IL-10 + CD19+ regulatory B cells(Breg) in patients with chronic hepatitis B(CHB).Methods 61 patients with CHB and 30 healthy controls(HCs)were selected.Peripheral blood mononuclear cells(PBMCs) were isolated from all subjects,and were stained with anti-IL-10-PE,anti-CD19-APC.Flow cytometry was adopted to analyze the expressions of IL-10 + CD19+ + Breg,and ELISA assay was used to analyze serum IL-10 level.Results Breg in CHB patients and HCs was 1.75％ (1.20％-2.55％),0.78％ (0.55％-1.40％),respectively,and the difference between the two groups was statistically significant (Z =-4.54,P ＜ 0.001).Breg in CHB patients with HBeAg positive was higher than that of negative(Z =-3.354,P =0.001).Levels of serum IL-10 in patients with CHB and HCs were 6.80 (5.41-8.42) pg/mL and 5.75 (4.40-6.54) pg/mL,and the difference was statistically significant(Z =-2.697,P =0.007).Serum IL-10 level was positively correlated with Breg in CHB patients (r =0.335,P =0.008).Conclusion Breg is obviously elevated in patients with CHB and may be affected by HBeAg.
