重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
4期
471-473
,共3页
牛少辉%张丽华%刘旭邦%陈杰
牛少輝%張麗華%劉旭邦%陳傑
우소휘%장려화%류욱방%진걸
催产素%再灌注损伤%一氧化氮%内皮素-1
催產素%再灌註損傷%一氧化氮%內皮素-1
최산소%재관주손상%일양화담%내피소-1
oxytocin%reperfusion injury%nitric oxide%endothelin1
目的:探讨缩宫素(OT)对大鼠急性心肌缺血再灌注后一氧化氮(NO)与内皮素-1(ET-1)的影响及可能的机制。方法将大鼠随机分为心肌缺血再灌注组(I/R组)、I/R+O T预处理组(I/R+O T组)、假手术组(S H组)和健康对照组(N组)。采用在体大鼠结扎冠状动脉前降支25 min后,松扎再灌注120 min形成I/R模型,SH组只穿线不结扎。I/R+ OT 组在缺血前25 min腹腔注射OT 0.03μg/kg ,其余3组给予等量生理盐水。再灌注结束后测定血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、NO及ET-1;再灌注结束时,采用HE染色进行心肌组织形态学分析。结果光镜下观察,N组与SH组心肌细胞排列整齐,无肌纤维破坏,I/R组心肌纤维排列不规则、断裂,心肌间质水肿,较多粒细胞灶状浸润,I/R+O T组可见心肌细胞排列、间质水肿及粒细胞浸润程度介于N组与SH组。与SH组相比,I/R组和I/R+ OT组血清CK-MB、LDH、ET-1升高,NO水平降低,差异均有统计学意义(P均<0.05),而N组的表达差异无统计学意义(P>0.05);与I/R组相比,I/R+OT组血清CK-MB、LDH、ET-1降低,NO水平升高,差异均有统计学意义(P均<0.05)。I/R组和I/R+OT组血清NO与ET-1水平呈负相关(P<0.05)。结论 OT预处理对大鼠I/R有保护作用,其作用机制可能与OT增加血清NO水平、降低ET-1水平有关。
目的:探討縮宮素(OT)對大鼠急性心肌缺血再灌註後一氧化氮(NO)與內皮素-1(ET-1)的影響及可能的機製。方法將大鼠隨機分為心肌缺血再灌註組(I/R組)、I/R+O T預處理組(I/R+O T組)、假手術組(S H組)和健康對照組(N組)。採用在體大鼠結扎冠狀動脈前降支25 min後,鬆扎再灌註120 min形成I/R模型,SH組隻穿線不結扎。I/R+ OT 組在缺血前25 min腹腔註射OT 0.03μg/kg ,其餘3組給予等量生理鹽水。再灌註結束後測定血清肌痠激酶同工酶(CK-MB)、乳痠脫氫酶(LDH)、NO及ET-1;再灌註結束時,採用HE染色進行心肌組織形態學分析。結果光鏡下觀察,N組與SH組心肌細胞排列整齊,無肌纖維破壞,I/R組心肌纖維排列不規則、斷裂,心肌間質水腫,較多粒細胞竈狀浸潤,I/R+O T組可見心肌細胞排列、間質水腫及粒細胞浸潤程度介于N組與SH組。與SH組相比,I/R組和I/R+ OT組血清CK-MB、LDH、ET-1升高,NO水平降低,差異均有統計學意義(P均<0.05),而N組的錶達差異無統計學意義(P>0.05);與I/R組相比,I/R+OT組血清CK-MB、LDH、ET-1降低,NO水平升高,差異均有統計學意義(P均<0.05)。I/R組和I/R+OT組血清NO與ET-1水平呈負相關(P<0.05)。結論 OT預處理對大鼠I/R有保護作用,其作用機製可能與OT增加血清NO水平、降低ET-1水平有關。
목적:탐토축궁소(OT)대대서급성심기결혈재관주후일양화담(NO)여내피소-1(ET-1)적영향급가능적궤제。방법장대서수궤분위심기결혈재관주조(I/R조)、I/R+O T예처리조(I/R+O T조)、가수술조(S H조)화건강대조조(N조)。채용재체대서결찰관상동맥전강지25 min후,송찰재관주120 min형성I/R모형,SH조지천선불결찰。I/R+ OT 조재결혈전25 min복강주사OT 0.03μg/kg ,기여3조급여등량생리염수。재관주결속후측정혈청기산격매동공매(CK-MB)、유산탈경매(LDH)、NO급ET-1;재관주결속시,채용HE염색진행심기조직형태학분석。결과광경하관찰,N조여SH조심기세포배렬정제,무기섬유파배,I/R조심기섬유배렬불규칙、단렬,심기간질수종,교다립세포조상침윤,I/R+O T조가견심기세포배렬、간질수종급립세포침윤정도개우N조여SH조。여SH조상비,I/R조화I/R+ OT조혈청CK-MB、LDH、ET-1승고,NO수평강저,차이균유통계학의의(P균<0.05),이N조적표체차이무통계학의의(P>0.05);여I/R조상비,I/R+OT조혈청CK-MB、LDH、ET-1강저,NO수평승고,차이균유통계학의의(P균<0.05)。I/R조화I/R+OT조혈청NO여ET-1수평정부상관(P<0.05)。결론 OT예처리대대서I/R유보호작용,기작용궤제가능여OT증가혈청NO수평、강저ET-1수평유관。
Objective To investigate the effect and possible mechanism of oxytocin on the expression of NO and ET-1 in precon-ditioning myocardium of rats with ischemia .Methods The rats were randomly divided into ischemic reperfusion group(I/R) ,ische-mic reperfusion + oxytocin pretreatment group(I/R+OT) ,sham operation group(SH) and nomal control group(N) .The myocar-dial ischemia-reperfusion model was induced by 25 min coronary occlusion and 120 min reperfusion in opened-chest anesthetized rats ,rats of SH group were not conducted with ligation but threading only .Group I/R+ OT were administered oxytocin(0 .03 g/kg ,i .p)25 min prior to ischemia ,the other three groups were given the same amount normal saline .After reperfusion ,nitric oxide (NO) ,endothelin-1(ET-1) ,creatin kinase isoenzyme(CK-MB)and lactate dehydrogenase(LDH)in blood serum were measured .At the end of reperfusion ,HE stain was used to observe the morphology of the cardiac muscular tissue .Results Pathological observa-tion showed that the myocardial cell in group SH and in X ranged in order ,with no fibers fractured ,myocardial fibers in I/R group were fractured and disorganized ,interstitial edema ,neutrophil infiltration were also observed .It was more serious in group I/R than in group I/R+OT .Compared with SH group ,the CK-MB ,LDH ,ET-1 of the I/R group and I/R+OT group increased ,the NO de-creased(P<0 .05) ,and it had no difference in group N and group SH (P>0 .05) Compared with the I/R group ,in I/R+OT group the expression of CK-MB ,LDH ,ET-1 were decreased ,the level of NO increased(P<0 .05) .There was a negative correlation be-tween NO and ET-1 in group I/R and I/R+ OT (P< 0 .05) .Conclusion Oxytocin pretreatment on rat myocardial ischemia and reperfusion has protective effect ,and its action mechanisms maybe associated with the increase of the content of NO and the de-crease of the content of ET-1 in blood serum .