CAJ | 학술논문

目的：通过高脂饲料诱发的SD大鼠肥胖模型研究肥胖对慢性前列腺炎（CP）发病的影响及其相关机制。方法实验分为普通饲料组、高脂饲料组、阳性对照组。通过喂养高脂饲料建立大鼠肥胖模型；前列腺液镜检检测各组大鼠卵磷脂小体密度和白细胞计数；采用ELISA检测前列腺液中炎症因子IL-6和 IL-8的分泌；实时定量-聚合酶链反应（RT-PCR）检测炎症相关基因IL-6和IL-8的表达水平。结果高脂饲料喂养大鼠5周后出现明显肥胖；高脂饲料组大鼠前列腺炎发病率明显高于普通饲料组，其前列腺液中IL-6和IL-8的分泌明显高于普通饲料组；高脂饲料组前列腺组织IL-6和IL-8的基因表达也明显升高。结论肥胖能够明显升高大鼠的前列腺组织炎症因子IL-6和IL-8的分泌和基因表达，从而导致CP发病率升高。
목적：통과고지사료유발적SD대서비반모형연구비반대만성전렬선염（CP）발병적영향급기상관궤제。방법실험분위보통사료조、고지사료조、양성대조조。통과위양고지사료건립대서비반모형；전렬선액경검검측각조대서란린지소체밀도화백세포계수；채용ELISA검측전렬선액중염증인자IL-6화 IL-8적분비；실시정량-취합매련반응（RT-PCR）검측염증상관기인IL-6화IL-8적표체수평。결과고지사료위양대서5주후출현명현비반；고지사료조대서전렬선염발병솔명현고우보통사료조，기전렬선액중IL-6화IL-8적분비명현고우보통사료조；고지사료조전렬선조직IL-6화IL-8적기인표체야명현승고。결론비반능구명현승고대서적전렬선조직염증인자IL-6화IL-8적분비화기인표체，종이도치CP발병솔승고。
Objective To investigate the effects of high fat diet induced obesity on rat prostatitis and assesses the metabolic fea-tures .Methods The normal diet group ,high-fat diet group and the positive control group were established according to the experi-mental requirements .Obesity model was established due to high-fat-diet feed .Microscopy was performed to analyze Lecithin density and leucocyte count .IL-6 and IL-8 Release level in the prostatic fluid were measured with ELISA (enzyme-linked immunosorbent as-say) kits according to the manufacturer′s directions .IL-6 and IL-8 mRNA levels of prostate were measured by real time PCR .Re-sults Compared with normal diet group ,high-fat diet group tended to be obesity obviously in 5 weeks of high-fat diet .The inci-dence rate of prostatitis in high fat diet group was significantly higher than that of control group ,and high-fat diet group had higher IL-6 and IL-8 releasing and mRNA levels .Conclusion High-fat diet could increase incidence of prostatitis in rat .The effect is par-tially due to IL-6 and IL-8 releasing and expression .