CAJ | 학술논문

目的探讨HLA-DRB等位基因与苏皖籍汉族人群甲真菌病的相关性.方法采用聚合酶链反应-序列特异性引物方法对50例红色毛癣菌甲真菌病患者、14例须毛癣菌甲真菌病患者和52例健康对照进行HLA-DRB等位基因分型.应用SPSS for windows 13.0软件包,采用X2检验比较甲真菌病患者组与对照组的HLA-DRB等位基因频率.结果红色毛癣菌甲真菌病患者HLA-DRB各等位基因频率与健康对照组相比差异均无统计学意义.须毛癣菌甲真菌病患者HLA-DRB1* 14等位基因频率为17.86％,较健康对照组(3.85％)升高(P＜ 0.01,OR=5.435,95％CI:1.353～21.835),HLA-DRB1*15频率为0,较健康对照组(16.3％)下降(P＜ 0.05,OR=0.837,95％ CI:0.768～0.911),差异均有统计学意义.结论 HLA-DRB等位基因可能与苏皖汉族人群甲红色毛癣菌感染无明显相关;HLA-DRB1*14可能是甲须毛癣菌感染的易感基因,而HLA-DRB1*15可能是甲须毛癣菌感染的拮抗基因;不同菌种感染所致的甲真菌病的遗传背景可能存在异质性.
목적 탐토HLA-DRB등위기인여소환적한족인군갑진균병적상관성.방법 채용취합매련반응-서렬특이성인물방법대50례홍색모선균갑진균병환자、14례수모선균갑진균병환자화52례건강대조진행HLA-DRB등위기인분형.응용SPSS for windows 13.0연건포,채용X2검험비교갑진균병환자조여대조조적HLA-DRB등위기인빈솔.결과 홍색모선균갑진균병환자HLA-DRB각등위기인빈솔여건강대조조상비차이균무통계학의의.수모선균갑진균병환자HLA-DRB1* 14등위기인빈솔위17.86％,교건강대조조(3.85％)승고(P＜ 0.01,OR=5.435,95％CI:1.353～21.835),HLA-DRB1*15빈솔위0,교건강대조조(16.3％)하강(P＜ 0.05,OR=0.837,95％ CI:0.768～0.911),차이균유통계학의의.결론 HLA-DRB등위기인가능여소환한족인군갑홍색모선균감염무명현상관;HLA-DRB1*14가능시갑수모선균감염적역감기인,이HLA-DRB1*15가능시갑수모선균감염적길항기인;불동균충감염소치적갑진균병적유전배경가능존재이질성.
Objective To analyze the association of HLA-DRB alleles with onychomycosis in Chinease Han population in Jiangsu and Auhui provinces.Methods Polymerase chain reaction with sequence-specific primers (PCR-SSP) was conducted to analyze the type of HLA-DRB alleles in 50 patients with Trichophyton rubruminduced onychomycosis,14 patients with Trichophyton mentagrophytes-induced onychomycosis and 52 healthy human controls of Chinese Han nationality.Data were processed with the SPSS 13.0 software for Windows.The frequency of HLA-DRB alleles was compared between these groups using chi-square test.Results No significant differences were observed in the frequency of HLA-DRB alleles between the patients with Trichophyton rubruminduced onychomycosis and healthy controls.The patients with Trichophyton mentagrophytes-induced onychomycosis showed significantly higher frequency of HLA-DRB1*14 allele (17.86％ vs.3.85％,P ＜ 0.01,OR =5.435,95％ CI:1.353-21.835),lower frequency of HLA-DRB1*15 allele (0 vs.16.3％,P ＜ 0.05,OR =0.837,95％ CI:0.768-0.911)compared with the healthy controls.Conclusions In Chinese Han population in Jiangsu and Anhui provinces,HLA-DRB alleles appear to be unassociated with Trichophyton rubrum-induced onychomycosis,whereas HLA-DRB1*14 allele seems to be a susceptible allele,and HLA-DRB1*15 be a protective allele,for Trichophyton mentagrophytes-induced onychomycosis.Genetic backgrounds may be different for onychomycosis caused by different Trichophyton species in Chinese Han population.