中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
5期
334-336
,共3页
张红梅%李成荣%刘勇军%刘威龙%付丹%徐六妹%谢靖婧%谭艳%王辉%陈心春%周伯平
張紅梅%李成榮%劉勇軍%劉威龍%付丹%徐六妹%謝靖婧%譚豔%王輝%陳心春%週伯平
장홍매%리성영%류용군%류위룡%부단%서륙매%사정청%담염%왕휘%진심춘%주백평
肠道病毒属%手足口病/病原学%序列分析
腸道病毒屬%手足口病/病原學%序列分析
장도병독속%수족구병/병원학%서렬분석
Enterovirus%Hand,foot and mouth disease/Etiology%Sequence analysis
目的 研究2008年深圳地区手足口病病原体EV71和CoxA16感染情况,为手足口病的防治提供依据.方法 应用RT-PCR技术检测307例手足口病患儿标本病原体EV71、CoxA16基因,将PCR产物测序,并应用ChstaIW2在线分析软件进行序列分析、进化树分析.结果 不同标本中EV71的阳性率分别为:大便标本24.4%(75/307)、咽拭子为7.8%(24/307)、外周血12.5%(1/8);CoxA16的阳性率分别为,大便13.8%(28/203),咽拭子11.0%(20/181),其中EV71和CoxA16同时为阳性的有3份(0.98%);脑脊液标本未检测到EV71和CoxA16.与标准株BrCr序列比对分析,测序的14例标本中,8例的EV71新出现多个位点变异,其中2例标本的A2528G变异导致氨基酸改变,即N595D,1例标本的A2714G变异引起氨基酸改变,即1658V.进化树分析显示,有2例标本的病毒株与anhui毒株亲缘关系较近,与标准株BrCr及深圳株SHH02-6、SHZH02-40、SHZH03-58等亲缘关系较远;而其余12株与上述毒株亲缘关系较远.根据文献报道的EV71基因分型方法分析,测序的14株EV71基因型为C型.结论 在引起2008年深圳手足口病病原体中相当的比例是EV71,且可能部分是anhui病毒株的入侵.
目的 研究2008年深圳地區手足口病病原體EV71和CoxA16感染情況,為手足口病的防治提供依據.方法 應用RT-PCR技術檢測307例手足口病患兒標本病原體EV71、CoxA16基因,將PCR產物測序,併應用ChstaIW2在線分析軟件進行序列分析、進化樹分析.結果 不同標本中EV71的暘性率分彆為:大便標本24.4%(75/307)、嚥拭子為7.8%(24/307)、外週血12.5%(1/8);CoxA16的暘性率分彆為,大便13.8%(28/203),嚥拭子11.0%(20/181),其中EV71和CoxA16同時為暘性的有3份(0.98%);腦脊液標本未檢測到EV71和CoxA16.與標準株BrCr序列比對分析,測序的14例標本中,8例的EV71新齣現多箇位點變異,其中2例標本的A2528G變異導緻氨基痠改變,即N595D,1例標本的A2714G變異引起氨基痠改變,即1658V.進化樹分析顯示,有2例標本的病毒株與anhui毒株親緣關繫較近,與標準株BrCr及深圳株SHH02-6、SHZH02-40、SHZH03-58等親緣關繫較遠;而其餘12株與上述毒株親緣關繫較遠.根據文獻報道的EV71基因分型方法分析,測序的14株EV71基因型為C型.結論 在引起2008年深圳手足口病病原體中相噹的比例是EV71,且可能部分是anhui病毒株的入侵.
목적 연구2008년심수지구수족구병병원체EV71화CoxA16감염정황,위수족구병적방치제공의거.방법 응용RT-PCR기술검측307례수족구병환인표본병원체EV71、CoxA16기인,장PCR산물측서,병응용ChstaIW2재선분석연건진행서렬분석、진화수분석.결과 불동표본중EV71적양성솔분별위:대편표본24.4%(75/307)、인식자위7.8%(24/307)、외주혈12.5%(1/8);CoxA16적양성솔분별위,대편13.8%(28/203),인식자11.0%(20/181),기중EV71화CoxA16동시위양성적유3빈(0.98%);뇌척액표본미검측도EV71화CoxA16.여표준주BrCr서렬비대분석,측서적14례표본중,8례적EV71신출현다개위점변이,기중2례표본적A2528G변이도치안기산개변,즉N595D,1례표본적A2714G변이인기안기산개변,즉1658V.진화수분석현시,유2례표본적병독주여anhui독주친연관계교근,여표준주BrCr급심수주SHH02-6、SHZH02-40、SHZH03-58등친연관계교원;이기여12주여상술독주친연관계교원.근거문헌보도적EV71기인분형방법분석,측서적14주EV71기인형위C형.결론 재인기2008년심수수족구병병원체중상당적비례시EV71,차가능부분시anhui병독주적입침.
Objective To investigate EV71 and CA16 pathogen of HFMD in Shenzhen in 2008,and to provide the evidence for the prevention and treatment HFMD.Method Using RT-PCR technology to detect the EV71 and CoxA16 genes of 307 samples HFMD;sequencing the purified PCR products from 14 samples.Using ClustaIW2 online analysis software for sequence and phylogenetic analysis of enterovirus 71.Result Percentnge of positive EV71 from different samples is shown as follows respectively:positive EV71 from stool samples is 24.4%(75/307),from throat swab-7.8%(24/307),from peripheral blood-12.5%(1/8).Percentage of positive CoxA16 is shown as follows respectively:positive EV71 from stool samples is 13.8%(28/203),from throat swab11.0%(20/181).Among all the 307 samples,three are positive for both EV71 and CoxA16.EV71 and CoxA16 are not detected in the samples of cerebrospinal fluid.Comparative analysis of nucleotide sequences of EV71 with those of strains BrCr and 11 deposited in GenBank demonstrated numerous disparities from 8 samples,but residue 595 from 2 samples and residue 658 from 1 sample are variable.The phylogenetic analysis based on VP1 region demonstrates that strains from 2 samples has the nearest genetic relationship with anhui strains,the farthest with BrCr and SHH02-6,SHZH02-40,SHZH03-58 strains,also strains from other 12 samples have the farthest genetic relationship with them.The genotypes A,B and C were classified as proposed by Brown et al.(1999).The EV71 from 14 samples were the member of genotype C.Conclusion EV71 among the pathogen of HFMD in Shenzhen in 2008 was majority.These EV71 may belong to the same genegroup with Anhui predominant strains.
