兰州交通大学学报
蘭州交通大學學報
란주교통대학학보
JOURNAL OF LANZHOU JIAOTONG UNIVERSITY（Natural Sciences）
2014年
3期
20-25
,共6页
张杰西%钟金魁%赵保卫%马锋锋%石夏颖%冉婧媛
張傑西%鐘金魁%趙保衛%馬鋒鋒%石夏穎%冉婧媛
장걸서%종금괴%조보위%마봉봉%석하영%염청원
高效液相色谱%芘%TX-100
高效液相色譜%芘%TX-100
고효액상색보%비%TX-100
High Performance Liquid Chromatography (HPLC)%pyrene%TX-100
建立表面活性剂与有机物共存体系中芘测定的高效液相色谱法．采用 C18反向色谱柱（2．1 mm×100 mm，5μm），通过调节和控制流动相、柱温、流速等因素，得到芘的优化测试条件．结果表明：流动相选用乙腈-水（80：20， V／V ），流速0．20 mL·min-1，柱温25℃是最优测试条件．同时，对 TX-100存在与不存在情况下芘的测试结果进行比较分析，使用 OriginPro 8．6对实验数据进行线性拟合，并进行统计学 t 检验，结果表明：X-100存在与否对水溶液中芘的测定没有显著影响．
建立錶麵活性劑與有機物共存體繫中芘測定的高效液相色譜法．採用 C18反嚮色譜柱（2．1 mm×100 mm，5μm），通過調節和控製流動相、柱溫、流速等因素，得到芘的優化測試條件．結果錶明：流動相選用乙腈-水（80：20， V／V ），流速0．20 mL·min-1，柱溫25℃是最優測試條件．同時，對 TX-100存在與不存在情況下芘的測試結果進行比較分析，使用 OriginPro 8．6對實驗數據進行線性擬閤，併進行統計學 t 檢驗，結果錶明：X-100存在與否對水溶液中芘的測定沒有顯著影響．
건립표면활성제여유궤물공존체계중비측정적고효액상색보법．채용 C18반향색보주（2．1 mm×100 mm，5μm），통과조절화공제류동상、주온、류속등인소，득도비적우화측시조건．결과표명：류동상선용을정-수（80：20， V／V ），류속0．20 mL·min-1，주온25℃시최우측시조건．동시，대 TX-100존재여불존재정황하비적측시결과진행비교분석，사용 OriginPro 8．6대실험수거진행선성의합，병진행통계학 t 검험，결과표명：X-100존재여부대수용액중비적측정몰유현저영향．
A new approach was established for the determination of pyrene in surfactants and or-ganics coexistent system by High Performance Liquid Chromatography (HPLC).The liquid chro-matography separation was carried on C18 column(2.1mm ×100mm,5μm).The optimized test conditions of pyrene in aqueous solution were determined by controlling the situations of mobile phase,column temperature,flow speed and so on.The results indicated that the optimal conditions were acetonitrile-water of 80:20(V/V ),flow speed of 0.20 mL·min-1 ,column temperature of 25℃.Besides,the experimental results of TX-100 were compared between the conditions of the presence and absence of pyrene,the data was linearly fitted by OriginPro.8.6 and t-test was also studied.The results show that no significant difference between the above conditions had been found.
