CAJ | 학술논문

旨在为甘蔗细胞色素P450还原酶基因的实验克隆、功能鉴定及其应用提供参考。本研究以甘蔗类似细胞色素P450还原酶基因的EST序列CF576130．1为探针，在甘蔗EST数据库中进行检索，并基于电子克隆技术获得了甘蔗细胞色素P450还原酶基因（Cytochrome P450 reductase）的一条cDNA全长序列，命名为ScCYP450。采用生物信息学方法，对该基因编码蛋白从氨基酸组成、理化性质、亚细胞定位、跨膜结构域、疏水性／亲水性、高级结构及功能域等方面进行预测和分析。结果表明该基因全长1821 bp，包含一个744 bp的开放阅读框，编码247个氨基酸，该基因编码蛋白定位于细胞内质网（膜），为可溶性碱性蛋白，不存在信号肽，二级结构原件多为无规卷曲，含有多个保守功能域，主要功能为辅酶因子生物合成和翻译功能。
지재위감자세포색소P450환원매기인적실험극륭、공능감정급기응용제공삼고。본연구이감자유사세포색소P450환원매기인적EST서렬CF576130．1위탐침，재감자EST수거고중진행검색，병기우전자극륭기술획득료감자세포색소P450환원매기인（Cytochrome P450 reductase）적일조cDNA전장서렬，명명위ScCYP450。채용생물신식학방법，대해기인편마단백종안기산조성、이화성질、아세포정위、과막결구역、소수성／친수성、고급결구급공능역등방면진행예측화분석。결과표명해기인전장1821 bp，포함일개744 bp적개방열독광，편마247개안기산，해기인편마단백정위우세포내질망（막），위가용성감성단백，불존재신호태，이급결구원건다위무규권곡，함유다개보수공능역，주요공능위보매인자생물합성화번역공능。
The present study aims to provide references for research on the experimental cloning, function identification and its application of sugarcane Cytochrome P450 reductase gene. The full-length cDNA sequence of one sugarcane Cytochrome P450 reductase gene termed ScCYP450, was obtained by in silico cloning using CF576130.1 sequence from sugarcane as the probe sequence. Using bioinformatics methods, several characteristics of ScCYP450 encoding amino acids, including the amino acid composition, physical and chemical properties, the subcellular localization, the transmembrane domain structure, hydrophobic/hydrophilic, advanced structure and forecast and plus the functional domains, were analyzed. The result showed that this gene is 1 821 bp length and contains a 744 bp open reading frame, encoding 247 amino acids, and the encoded protein localizes in cells endoplasmic reticulum ( film) , as the alkaline soluble protein. There is no signal peptide. The secondary structure mostly consistes of random coil. ScCYP450 protein contains several conservative functional domains, and the main function of this protein involved in growth factor biosynthesis and transcription.