CAJ | 학술논문

目的：采用 HPLC法同时测定腰腿痛丸中麻黄碱、士的宁和马钱子碱的含量。方法采用 HPLC法。色谱柱为安捷伦C18色谱柱（250 mm ×4．6 mm ，5μm）；流动相为乙腈-1 mL · L -1磷酸（12∶88）；流速为1．0 mL · min-1；检测波长为210和254 nm ；柱温为30℃。结果麻黄碱在1．0～25μg·mL -1范围内线性关系良好（r＝0．9999），平均回收率为98．7％；士的宁在2．0～50μg·mL -1范围内线性关系良好（r＝0．9998），平均回收率为99．1％；马钱子碱在2～50μg·mL -1范围内线性关系良好（r＝0．9999），平均回收率为97．9％。3批腰腿痛丸中麻黄碱、士的宁及马钱子碱的平均含量分别为93．1，106．7和76．8μg · g -1。结论该方法灵敏，精密度好，结果准确可靠。
목적：채용 HPLC법동시측정요퇴통환중마황감、사적저화마전자감적함량。방법채용 HPLC법。색보주위안첩륜C18색보주（250 mm ×4．6 mm ，5μm）；류동상위을정-1 mL · L -1린산（12∶88）；류속위1．0 mL · min-1；검측파장위210화254 nm ；주온위30℃。결과마황감재1．0～25μg·mL -1범위내선성관계량호（r＝0．9999），평균회수솔위98．7％；사적저재2．0～50μg·mL -1범위내선성관계량호（r＝0．9998），평균회수솔위99．1％；마전자감재2～50μg·mL -1범위내선성관계량호（r＝0．9999），평균회수솔위97．9％。3비요퇴통환중마황감、사적저급마전자감적평균함량분별위93．1，106．7화76．8μg · g -1。결론해방법령민，정밀도호，결과준학가고。
Objective To establish an HPLC method for the simultaneous determination of ephedrine ,strychnine and brucine in Yaotuitong Pills .Methods The samples were separated on an Agilent C18 (250 mm × 4 .6 mm ,5μm) at 30 ℃ and detected at 210 and 254 nm .The mobile phase was consisted of acetonitrile-1 mL · L -1 phosphoric acid (12 ∶ 88 ) with a flow rate of 1 .0 mL · min-1 . Results The linear ranges of ephedrine ,strychnine and brucine were 1 .0-25 μg · mL -1 (r=0 .999 9) ,2 .0-50 μg · mL -1 (r=0 .999 8) ,2 .0-50 μg · mL -1 (r=0 .999 9) and the average recoveries were 98 .7% ,99 .1% and 97 .9% .The average contents of ephedrine ,strychnine and brucine in three batches of Yaotuitong Pills were 93 .1 ,106 .7 and 76 .8μg · g -1 .Conclusion The meth-od is sensitive ,accurate ,and can be used to control the quality of Yaotuitong Pills .