西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
4期
361-363
,共3页
田园%王海亮%解笑瑜%王嗣岑
田園%王海亮%解笑瑜%王嗣岑
전완%왕해량%해소유%왕사잠
和血明目片%黄芩苷%高效液相色谱法%含量测定
和血明目片%黃芩苷%高效液相色譜法%含量測定
화혈명목편%황금감%고효액상색보법%함량측정
Hexuemingmu Tablets%baicalin%HPLC%content determination
目的：建立和血明目片中黄芩苷的 H PLC含量测定方法。方法采用反相高效液相色谱法。C18色谱柱；流动相为甲醇-磷酸-水溶液（47∶0．2∶53）；检测波长为280 nm ；流速为1．0 mL · min-1；柱温30℃；峰面积外标法定量。结果黄芩苷在0．0262～0．3936 mg · mL -1范围内呈良好的线性关系，平均回收率96．3％，RSD为1．6％（n＝6）。结论该方法简便，结果准确可靠，可作为该制剂的质量控制方法。
目的：建立和血明目片中黃芩苷的 H PLC含量測定方法。方法採用反相高效液相色譜法。C18色譜柱；流動相為甲醇-燐痠-水溶液（47∶0．2∶53）；檢測波長為280 nm ；流速為1．0 mL · min-1；柱溫30℃；峰麵積外標法定量。結果黃芩苷在0．0262～0．3936 mg · mL -1範圍內呈良好的線性關繫，平均迴收率96．3％，RSD為1．6％（n＝6）。結論該方法簡便，結果準確可靠，可作為該製劑的質量控製方法。
목적：건립화혈명목편중황금감적 H PLC함량측정방법。방법채용반상고효액상색보법。C18색보주；류동상위갑순-린산-수용액（47∶0．2∶53）；검측파장위280 nm ；류속위1．0 mL · min-1；주온30℃；봉면적외표법정량。결과황금감재0．0262～0．3936 mg · mL -1범위내정량호적선성관계，평균회수솔96．3％，RSD위1．6％（n＝6）。결론해방법간편，결과준학가고，가작위해제제적질량공제방법。
Objective To establish an HPLC method for the determination of baicalin in Hexuemingmu Tablets . Methods The HPLC method was carried out on a C18 column using methanol-phosphoric acid-water (47∶0 .2∶53) as mobile phase .The detec-tion wavelength was 280 nm ,the flow rate was 1 .0 mL · min-1 ,and the temperature of column was 30 ℃ .The baicalin in Hex-uemingmu Tablets was determined by HPLC-peak area external standard method .Results The calibration curve for baicalin was linear in the range of 0 .026 2-0 .393 6 mg · mL -1 .The average recovery was 96 .3% with RSD of 1 .6% (n=6) .Conclusion The method is simple ,precise and reliable for determining the content of baicalin in Hexuemingmu Tablets .
