CAJ | 학술논문

目的：以原儿茶酸和原儿茶醛为指标筛选丁香叶的提取工艺。方法以原儿茶醛及原儿茶酸为指标，采用单因素分析考察丁香叶提取工艺中的溶剂倍数、提取时间和回流次数，HPLC法测定原儿茶醛和原儿茶酸，色谱条件为色谱柱：Planetsil C18（150 mm ×4．6 mm ，5μm）；流动相：甲醇-水-冰醋酸（12∶78∶0．5）；流速：1．0 mL · min-1；检测波长：280 nm ；柱温：30℃。结果丁香叶用100倍水回流提取3次（2h，2h，1h），提取率最高。丁香叶中原儿茶醛和原儿茶酸含量分别为20．7～23．1μg·g -1和83．3～92．4μg · g -1。结论该研究获得一种提取率相对较高的方法以提取丁香叶中活性成分。
목적：이원인다산화원인다철위지표사선정향협적제취공예。방법이원인다철급원인다산위지표，채용단인소분석고찰정향협제취공예중적용제배수、제취시간화회류차수，HPLC법측정원인다철화원인다산，색보조건위색보주：Planetsil C18（150 mm ×4．6 mm ，5μm）；류동상：갑순-수-빙작산（12∶78∶0．5）；류속：1．0 mL · min-1；검측파장：280 nm ；주온：30℃。결과정향협용100배수회류제취3차（2h，2h，1h），제취솔최고。정향협중원인다철화원인다산함량분별위20．7～23．1μg·g -1화83．3～92．4μg · g -1。결론해연구획득일충제취솔상대교고적방법이제취정향협중활성성분。
Objective To establish an extraction process of folium syringae with protocatechuic acid and protocatechuic aldehyde as the index components .Methods The solvent ratios ,extraction time and the reflux counts were evaluated .An HPLC system was used to determine the contents of protocatechuic acid and protocatechuic aldehyde .The chromatographic conditions were as fol-lows :planetsil C18 (150 mm × 4 .6 mm ,5μm) was utilized with a mixture of methanol-water-glacial acetic acid (12∶78∶0 .5) as the mobile phase ,the flow rate was 1 .0 mL · min-1 ,the detection wavelength was 280 nm ,and the column temperature was set at 30 ℃ .Results The highest extraction rate was obtained after three extractions (2 ,2 ,1 h) with 100 folds of water .The con-tents of protocatechuic acid and protocatechuic aldehyde were ranged from 20 .7 to 23 .1 μg · g -1 and 83 .3 to 92 .4 μg · g -1 ,re-spectively .Conclusion An extraction method with relatively high extraction rate was established .The method could be utilized to extract the bioactive constituents from folium syringae .