西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
4期
350-353
,共4页
喜树碱%响应曲面法%提取工艺
喜樹堿%響應麯麵法%提取工藝
희수감%향응곡면법%제취공예
camptothecin%response surface methodology%extraction technique
目的：用响应曲面法优选喜树碱的超声提取工艺。方法以提取时间、提取温度和料液比为考察因素，用 HPLC-DAD法测定喜树碱的含量，通过响应曲面法优化喜树碱超声提取工艺。结果最佳提取工艺为提取温度49℃，提取时间2．5h，料液比1∶17。结论响应曲面法筛选的喜树碱提取工艺稳定、可行。
目的：用響應麯麵法優選喜樹堿的超聲提取工藝。方法以提取時間、提取溫度和料液比為攷察因素，用 HPLC-DAD法測定喜樹堿的含量，通過響應麯麵法優化喜樹堿超聲提取工藝。結果最佳提取工藝為提取溫度49℃，提取時間2．5h，料液比1∶17。結論響應麯麵法篩選的喜樹堿提取工藝穩定、可行。
목적：용향응곡면법우선희수감적초성제취공예。방법이제취시간、제취온도화료액비위고찰인소，용 HPLC-DAD법측정희수감적함량，통과향응곡면법우화희수감초성제취공예。결과최가제취공예위제취온도49℃，제취시간2．5h，료액비1∶17。결론향응곡면법사선적희수감제취공예은정、가행。
Objective To optimize the extraction technology of camptothecin (CPT) by response surface methodology(RSM) .Meth-od RSM was used to optimize the extraction process of CPT by optimizing the extraction temperature ,extraction time and ratio of material to liquid .The content of CPT was determined by HPLC-DAD .Results The optimal extraction parameters were as fol-lows :extraction temperature 49 ℃ ,extraction time 2 .5 h and the ratio of material to liquid 1∶17 .Conclusion The established ex-traction technology is stable and feasible .
