CAJ | 학술논문

提出一种新型可重复实现单细胞的RT-PCR的实验技术，扩增目的基因。应用玻璃微电极提取单个神经细胞，裂解获得mRNA，利用RT-PCR技术检测单个神经细胞的离子通道等基因的表达。应用此技术可以灵敏地检测特定电生理现象的分子生物学基础。此法可以比较大鼠背根神经节中大细胞内 HCN1的mRNA表达量。实验结果表明：扩增产物的量足够用于后续实验，而且3个重复的均一性也很好，充分表明了该研究提出的实验技术的可行性。
제출일충신형가중복실현단세포적RT-PCR적실험기술，확증목적기인。응용파리미전겁제취단개신경세포，렬해획득mRNA，이용RT-PCR기술검측단개신경세포적리자통도등기인적표체。응용차기술가이령민지검측특정전생리현상적분자생물학기출。차법가이비교대서배근신경절중대세포내 HCN1적mRNA표체량。실험결과표명：확증산물적량족구용우후속실험，이차3개중복적균일성야흔호，충분표명료해연구제출적실험기술적가행성。
It is very difficult to complete the application of RT-PCR based on a single cell because of the low level of mRNA.This study is to explore a novel experimental technique to achieve a repeatable process of the single cell RT-PCR,which could be used to characterize the biophysical properties of ion channels genes in a single neuron. The single cell was picked by a glass micropipette,followed by a standard RT-PCR to explore the expression levels of ion channel genes.Single-cell RT-PCR could serve as a precise detector to analyze the molecular basis of the special electrophysiological characteristics. Using the method proposed in the article can be repeated to obtain the expression of HCN1 and HPRT in the big cell of rat dorsal root ganglion. It is enough to continues the next experiment,which proves the feasibility of this technique.