CAJ | 학술논문

从噬菌体随机十二肽库筛选出两个与人胃腺癌细胞 SGC-7901表面特异结合的阳性噬菌体克隆，用细胞免疫荧光法鉴定这些克隆与SGC-7901结合的特异性，据亲和力确定克隆GSP5为最佳克隆，进一步以免疫细胞／组织化学等方法鉴定此克隆与胃癌细胞和临床组织的特异性／敏感性。结果显示，噬菌体克隆GSP5对SGC-7901细胞及胃癌临床组织具有较好的结合特异性／敏感性。该多肽有望成为胃癌分子影像诊断与靶向治疗的候选多肽导向分子。
종서균체수궤십이태고사선출량개여인위선암세포 SGC-7901표면특이결합적양성서균체극륭，용세포면역형광법감정저사극륭여SGC-7901결합적특이성，거친화력학정극륭GSP5위최가극륭，진일보이면역세포／조직화학등방법감정차극륭여위암세포화림상조직적특이성／민감성。결과현시，서균체극륭GSP5대SGC-7901세포급위암림상조직구유교호적결합특이성／민감성。해다태유망성위위암분자영상진단여파향치료적후선다태도향분자。
Using phage displayed peptides library screening method,we previously screened out two peptides specifically binding to gastric carcinoma cell SGC-7901.The specific affinity of GSP5 clone was confirmed as best using immunofluorescence assays.Then,the specific affinities of GSP5 clone to the cells and tissues of gastric cancer were further confirmed using different immunofluorescence assays under different microscopes.The results show that the peptide clone GSP5 binds to the cells and clinical tissues of gastric cancer at high affinity.Therefore,the clone GSP5 is of the potential to be used as a peptide probe for the early diagnosis and targeting therapy of gastric cancer.