南通大学学报（医学版）
南通大學學報（醫學版）
남통대학학보（의학판）
JOURNAL OF NANTONG UNIVERSITY(MEDICAL SCIENCES)
2014年
4期
263-266,267
,共5页
刘培根%朱建伟%马利林
劉培根%硃建偉%馬利林
류배근%주건위%마리림
大肠癌%尿激酶型纤溶酶原激活剂受体%小片段干扰核糖核酸%细胞增殖%细胞迁移
大腸癌%尿激酶型纖溶酶原激活劑受體%小片段榦擾覈糖覈痠%細胞增殖%細胞遷移
대장암%뇨격매형섬용매원격활제수체%소편단간우핵당핵산%세포증식%세포천이
colorectal cancer%urokinase-type plasmino gen activator receptor%small interfering RNA%cell proliferation%cell migration
目的：通过uPAR特异siRNA靶向下调大肠癌LOVO细胞uPAR的表达，研究大肠癌细胞恶性生物学行为的变化。方法：构建3对uPAR特异siRNA，将其转入大肠癌LOVO细胞，用Western Blot检测uPAR-siRNA干扰效果，选择干扰效率最高的一对siRNA进行后续实验。利用流式细胞术及CCK-8试验检测uPAR-siRNA转染后大肠癌细胞增殖变化，利用Transwell小室实验及划痕实验检测uPAR-siRNA转染后对大肠癌细胞迁移能力变化。结果：大肠癌细胞分别转染3对uPAR-siRNA后，uPAR的表达明显下降，其中uPAR-siRNA-3的干扰效率最高；大肠癌细胞转染uPAR-siRNA-3后，G1期细胞数量明显增加，细胞增殖能力明显下降，细胞迁移数量明显减少及细胞迁移距离明显下降(均P<0.05)。结论：uPAR特异siRNA转染大肠癌细胞LOVO后，明显下调uPAR的表达；uPAR-siRNA转染大肠癌细胞LOVO后，明显抑制大肠癌细胞的增殖和迁移能力。
目的：通過uPAR特異siRNA靶嚮下調大腸癌LOVO細胞uPAR的錶達，研究大腸癌細胞噁性生物學行為的變化。方法：構建3對uPAR特異siRNA，將其轉入大腸癌LOVO細胞，用Western Blot檢測uPAR-siRNA榦擾效果，選擇榦擾效率最高的一對siRNA進行後續實驗。利用流式細胞術及CCK-8試驗檢測uPAR-siRNA轉染後大腸癌細胞增殖變化，利用Transwell小室實驗及劃痕實驗檢測uPAR-siRNA轉染後對大腸癌細胞遷移能力變化。結果：大腸癌細胞分彆轉染3對uPAR-siRNA後，uPAR的錶達明顯下降，其中uPAR-siRNA-3的榦擾效率最高；大腸癌細胞轉染uPAR-siRNA-3後，G1期細胞數量明顯增加，細胞增殖能力明顯下降，細胞遷移數量明顯減少及細胞遷移距離明顯下降(均P<0.05)。結論：uPAR特異siRNA轉染大腸癌細胞LOVO後，明顯下調uPAR的錶達；uPAR-siRNA轉染大腸癌細胞LOVO後，明顯抑製大腸癌細胞的增殖和遷移能力。
목적：통과uPAR특이siRNA파향하조대장암LOVO세포uPAR적표체，연구대장암세포악성생물학행위적변화。방법：구건3대uPAR특이siRNA，장기전입대장암LOVO세포，용Western Blot검측uPAR-siRNA간우효과，선택간우효솔최고적일대siRNA진행후속실험。이용류식세포술급CCK-8시험검측uPAR-siRNA전염후대장암세포증식변화，이용Transwell소실실험급화흔실험검측uPAR-siRNA전염후대대장암세포천이능력변화。결과：대장암세포분별전염3대uPAR-siRNA후，uPAR적표체명현하강，기중uPAR-siRNA-3적간우효솔최고；대장암세포전염uPAR-siRNA-3후，G1기세포수량명현증가，세포증식능력명현하강，세포천이수량명현감소급세포천이거리명현하강(균P<0.05)。결론：uPAR특이siRNA전염대장암세포LOVO후，명현하조uPAR적표체；uPAR-siRNA전염대장암세포LOVO후，명현억제대장암세포적증식화천이능력。
Objective: To investigate the change of malignant behaviors in colorectal cancer cells(LOVO) through targeted down-regulation the expression of uPAR by the specific uPAR-siRNA. Methods: Three pairs of specific uPAR-siRNA were constructed and transfected into LOVO colorectal cancer cell line. The interference efficiencies of uPAR-siRNAs were detected by Western Blot. The uPAR-siRNA with the highest interference efficiency was selected for the following experiments. The proliferation of colorectal cancer cells after transfection with uPAR-siRNA was detected by flow cytometry and CCK-8 assay. The migration of colorectal cancer cells after transfection with uPAR-siRNA was determinated by Transwell chamber system and Wound healing assay. Results: The expressions of uPAR in the colorectal cancer cells were significantly down-regulated after transfection with uPAR-siRNAs, and uPAR-siRNA-3 caused the most reduction of uPAR. After transfection with uPAR-siRNA-3 into colorectal cancer cells, the number in G1 phase was significantly increased, but the abilities of proliferation and migration were significantly inhibited , compared with the control group (all P<0.05). Conclusions: The expression of uPAR in colorectal cancer cells after transfection with the specific siRNAs of uPAR is significantly down-regulated . Targeted down-regulation of uPAR inhibits the malignant biological behaviors including proliferation and migration of colorectal cancer cells.
