CAJ | 학술논문

目的：探讨拮抗线粒体穿梭系统关键分子肉毒碱对肝脂肪氧化的影响。方法：野生型C57BJ/6j (wt/wt)鼠腹腔注射0.05%(W/W/d)肉毒碱相似物[3-(2，2，2-trimethyl hydrazinium) propionate dihydrate，THP]拮抗循环肉毒碱，对照鼠以等量生理盐水(0.85%，W/V)注射，2周后以乙醚麻醉处死留取肝组织；油红O染色观察肝组织脂肪积聚，从肝组织提取并以放射法分析长、短链脂酰肉毒碱，游离及总肉毒碱浓度。结果：THP 注射后鼠肝脏质量明显高于对照组(P<0.05)，油红O染色证实模型鼠肝组织出现大量脂肪积聚，高于对照鼠的5~6倍；肝组织总、长链和游离肉毒碱比浓度(nmol/g湿重肝组织)显著高于对照组(P<0.05)，分别为(392.16±53.08) vs (323.78±51.29)、(78.32±9.51) vs (65.80±7.73)和(252.28±28.21) vs (151.46±37.81)，短链脂酰肉毒碱比浓度明显低于对照组(61.56±23.28 vs 106.92±41.16)。结论：肉毒碱是保证脂肪酸进行β-氧化关键分子和代谢的重要因素。
목적：탐토길항선립체천사계통관건분자육독감대간지방양화적영향。방법：야생형C57BJ/6j (wt/wt)서복강주사0.05%(W/W/d)육독감상사물[3-(2，2，2-trimethyl hydrazinium) propionate dihydrate，THP]길항순배육독감，대조서이등량생리염수(0.85%，W/V)주사，2주후이을미마취처사류취간조직；유홍O염색관찰간조직지방적취，종간조직제취병이방사법분석장、단련지선육독감，유리급총육독감농도。결과：THP 주사후서간장질량명현고우대조조(P<0.05)，유홍O염색증실모형서간조직출현대량지방적취，고우대조서적5~6배；간조직총、장련화유리육독감비농도(nmol/g습중간조직)현저고우대조조(P<0.05)，분별위(392.16±53.08) vs (323.78±51.29)、(78.32±9.51) vs (65.80±7.73)화(252.28±28.21) vs (151.46±37.81)，단련지선육독감비농도명현저우대조조(61.56±23.28 vs 106.92±41.16)。결론：육독감시보증지방산진행β-양화관건분자화대사적중요인소。
Objectiv e: To explore antagonistic key molecule of mitochondrial carnitine shuttle system on effects of hepatic fatty acid metabolism. Methods: Wild type C57BJ/6j(wt/wt) mice were intraperitoneally injected 0.05% of carnitine analogue [(3-(2,2,2-trimethyl hydrazinium, W/W/d) propionate dehydrate,THP)] to antagonistic carnitine, and the control mice with equal quantity saline (0.85%, W/V), and sacrificed with ether anesthesia after two weeks, and the livers were strained with oil red for observing hepatic fatty accumulation and the levels of different type carnitines including total, free, long, and short-chain were quantitatively analyzed in liver tissues by radiometric method. Results: After the mice injected with THP, the liver weight in the injected group was significantly increased(P<0.05) than that in the control group, with the fatty accu-mulation in hepatocytes. The hepatic specific concentrations(nmol/g wet liver) in the injected group were significantly higher (P<0.05) than those in the control, with (392.16±53.08) vs (323.78±51.29) in total, (252.28±28.21) vs (151.46±37.81) in free, and (78.32±9.51) vs (65.80±7.73) in long chain carnitine, and remarkedly decreased in short-chain carnitine (61.56±23.28 vs 106.92±41.16), respectively. Conclusion: Carnitine is located on inner membrance of mitochondria and one of key enzymes limited β-oxidation process.