中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
6期
1098-1102,1109
,共6页
陈林林%桂春%韦晓敏%陈力铨%朱立光
陳林林%桂春%韋曉敏%陳力銓%硃立光
진림림%계춘%위효민%진력전%주립광
血管内皮生长因子%血管生成素%内皮抑素%糖尿病心肌病%大鼠
血管內皮生長因子%血管生成素%內皮抑素%糖尿病心肌病%大鼠
혈관내피생장인자%혈관생성소%내피억소%당뇨병심기병%대서
Vascular endothelial growth factor%Angiopoietins%Endostatin%Diabetic cardiomyopathies%Rats
目的：检测糖尿病大鼠心肌组织中血管生成因子的表达。方法：选择雄性SD大鼠一次性腹腔注射链脲佐菌素制造1型糖尿病模型，糖尿病成功诱导后12周，运用MPA心功能分析系统评估心功能改变，Masson染色计算心肌胶原容积分数（collagen volume fraction，CVF），CD31免疫组织化学法测定心肌毛细血管并计算心肌毛细血管／心肌细胞比值（capillary／myocyte，C／M），Western blotting 检测血管内皮生成因子（VEGF）、血管生成素1（angiopoietin-1，Ang-1）、血管生成素2（angiopoietin-2，Ang-2）和内皮抑素（endostatin）在心肌组织中的表达。结果：与正常对照组比较，糖尿病大鼠左心室舒张末期压力（ LVEDP ）明显增高（ P＜0.01），而左心室压力上升最大速率（＋dp／dtmax）和左心室压力下降最大速率（-dp／dtmax）均明显下降（P＜0．05）；心肌C／M比值、 VEGF和Ang-1表达明显减少（P＜0．05）；而心肌CVF含量和endostatin表达显著增高（P＜0．05），但Ang-2表达无明显差异。结论：VEGF和Ang-1在糖尿病大鼠心肌组织表达下调，endostatin表达显著上调，可能是糖尿病心肌病发生的重要机制。
目的：檢測糖尿病大鼠心肌組織中血管生成因子的錶達。方法：選擇雄性SD大鼠一次性腹腔註射鏈脲佐菌素製造1型糖尿病模型，糖尿病成功誘導後12週，運用MPA心功能分析繫統評估心功能改變，Masson染色計算心肌膠原容積分數（collagen volume fraction，CVF），CD31免疫組織化學法測定心肌毛細血管併計算心肌毛細血管／心肌細胞比值（capillary／myocyte，C／M），Western blotting 檢測血管內皮生成因子（VEGF）、血管生成素1（angiopoietin-1，Ang-1）、血管生成素2（angiopoietin-2，Ang-2）和內皮抑素（endostatin）在心肌組織中的錶達。結果：與正常對照組比較，糖尿病大鼠左心室舒張末期壓力（ LVEDP ）明顯增高（ P＜0.01），而左心室壓力上升最大速率（＋dp／dtmax）和左心室壓力下降最大速率（-dp／dtmax）均明顯下降（P＜0．05）；心肌C／M比值、 VEGF和Ang-1錶達明顯減少（P＜0．05）；而心肌CVF含量和endostatin錶達顯著增高（P＜0．05），但Ang-2錶達無明顯差異。結論：VEGF和Ang-1在糖尿病大鼠心肌組織錶達下調，endostatin錶達顯著上調，可能是糖尿病心肌病髮生的重要機製。
목적：검측당뇨병대서심기조직중혈관생성인자적표체。방법：선택웅성SD대서일차성복강주사련뇨좌균소제조1형당뇨병모형，당뇨병성공유도후12주，운용MPA심공능분석계통평고심공능개변，Masson염색계산심기효원용적분수（collagen volume fraction，CVF），CD31면역조직화학법측정심기모세혈관병계산심기모세혈관／심기세포비치（capillary／myocyte，C／M），Western blotting 검측혈관내피생성인자（VEGF）、혈관생성소1（angiopoietin-1，Ang-1）、혈관생성소2（angiopoietin-2，Ang-2）화내피억소（endostatin）재심기조직중적표체。결과：여정상대조조비교，당뇨병대서좌심실서장말기압력（ LVEDP ）명현증고（ P＜0.01），이좌심실압력상승최대속솔（＋dp／dtmax）화좌심실압력하강최대속솔（-dp／dtmax）균명현하강（P＜0．05）；심기C／M비치、 VEGF화Ang-1표체명현감소（P＜0．05）；이심기CVF함량화endostatin표체현저증고（P＜0．05），단Ang-2표체무명현차이。결론：VEGF화Ang-1재당뇨병대서심기조직표체하조，endostatin표체현저상조，가능시당뇨병심기병발생적중요궤제。
AIM:To observe the expression of angiogenesis factors in the myocardial tissue of streptozotocin-induced diabetic rats .METHODS:The diabetic rat model was induced by intraperitoneal injection of streptozotocin .After 12 weeks, the cardiac function was measured by MPA cardiac function analysis system .The myocardial collagen volume fraction ( CVF) was assessed by Masson staining .The capillary vessels was quantified as the ratio of capillary to myocyte (C/M) using CD31 immunostaining.The expression levels of vascular endothelial growth factor (VEGF), angiopoietin ( Ang)-1, endostatin and Ang-2 were observed by Western blotting .RESULTS:Compared with normal control group , the left ventricular end-diastolic pressure (LVEDP) was evidently increased (P<0.01), but left ventricular pressure rise maximum rate (+dp/dtmax), left ventricular pressure decrease maximum rate (-dp/dtmax) and the ratio of capillary/myo-cyte (C/M) were significantly decreased (P<0.05).The CVF and the expression level of endostatin were significantly increased, whereas the expression levels of VEGF and Ang-1 evidently decreased (both P<0.05) in diabetic rats.Howev-er, no marked difference in the expression of Ang-2 between the 2 groups was observed (P>0.05).CONCLUSION:Im-balances between the angiogenic factors (VEGF and Ang-1) and anti-angiogenic factors (endostatin) may play an impor-tant role in the pathogenesis of diabetic cardiomyopathy .
