中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
6期
1114-1118
,共5页
叶莉莎%韩园%刘启星%张占琴%梅虹霞%曹红%连庆泉%李军
葉莉莎%韓園%劉啟星%張佔琴%梅虹霞%曹紅%連慶泉%李軍
협리사%한완%류계성%장점금%매홍하%조홍%련경천%리군
姜黄素%阿尔茨海默病%学习%记忆%高迁移率族框蛋白1%c-Jun氨基末端激酶
薑黃素%阿爾茨海默病%學習%記憶%高遷移率族框蛋白1%c-Jun氨基末耑激酶
강황소%아이자해묵병%학습%기억%고천이솔족광단백1%c-Jun안기말단격매
Curcumin%Alzheimer disease%Learning%Memory%High mobility group box protein 1%c-Jun N-terminal kinase
目的：探讨姜黄素对阿尔茨海默病（AD）大鼠学习记忆能力、海马高迁移率族框蛋白1（HMGB1）及c-Jun氨基末端激酶（ JNK）表达的影响。方法：36只雄性SD大鼠随机分为4组：空白对照组（ A组）、AD模型组（B组，鹅膏覃氨酸Meynert基底核注射）、AD模型＋姜黄素治疗组（C组，腹腔注射100 mg· kg-1· d-1持续6 d）和AD模型＋溶剂对照组（ D组，腹腔注射4 mL／kg玉米油持续6 d）。 Morris水迷宫测试大鼠平均逃避潜伏期（AEL），免疫组化和Western blotting法检测海马HMGB1和JNK的表达。结果：与A组相比，B和D组大鼠造模后各时点AEL明显延长（P＜0．05），C组大鼠第5天和第6天 AEL较B组相应时点明显缩短（P＜0．05）；B组和D组海马存在HMGB1大量胞浆、胞外释放情况（P＜0．05），而C组海马区HMGB1胞浆、胞外释放无明显差异（P＞0.05）。4组大鼠海马总HMGB1表达水平比较差异无统计学意义（P＞0．05），但B组和D组海马JNK表达水平增高（P＜0．05）。结论：姜黄素能够改善AD大鼠的学习记忆能力，其机制可能与抑制HMGB1的胞浆、胞外释放及JNK的表达下调有关。
目的：探討薑黃素對阿爾茨海默病（AD）大鼠學習記憶能力、海馬高遷移率族框蛋白1（HMGB1）及c-Jun氨基末耑激酶（ JNK）錶達的影響。方法：36隻雄性SD大鼠隨機分為4組：空白對照組（ A組）、AD模型組（B組，鵝膏覃氨痠Meynert基底覈註射）、AD模型＋薑黃素治療組（C組，腹腔註射100 mg· kg-1· d-1持續6 d）和AD模型＋溶劑對照組（ D組，腹腔註射4 mL／kg玉米油持續6 d）。 Morris水迷宮測試大鼠平均逃避潛伏期（AEL），免疫組化和Western blotting法檢測海馬HMGB1和JNK的錶達。結果：與A組相比，B和D組大鼠造模後各時點AEL明顯延長（P＜0．05），C組大鼠第5天和第6天 AEL較B組相應時點明顯縮短（P＜0．05）；B組和D組海馬存在HMGB1大量胞漿、胞外釋放情況（P＜0．05），而C組海馬區HMGB1胞漿、胞外釋放無明顯差異（P＞0.05）。4組大鼠海馬總HMGB1錶達水平比較差異無統計學意義（P＞0．05），但B組和D組海馬JNK錶達水平增高（P＜0．05）。結論：薑黃素能夠改善AD大鼠的學習記憶能力，其機製可能與抑製HMGB1的胞漿、胞外釋放及JNK的錶達下調有關。
목적：탐토강황소대아이자해묵병（AD）대서학습기억능력、해마고천이솔족광단백1（HMGB1）급c-Jun안기말단격매（ JNK）표체적영향。방법：36지웅성SD대서수궤분위4조：공백대조조（ A조）、AD모형조（B조，아고담안산Meynert기저핵주사）、AD모형＋강황소치료조（C조，복강주사100 mg· kg-1· d-1지속6 d）화AD모형＋용제대조조（ D조，복강주사4 mL／kg옥미유지속6 d）。 Morris수미궁측시대서평균도피잠복기（AEL），면역조화화Western blotting법검측해마HMGB1화JNK적표체。결과：여A조상비，B화D조대서조모후각시점AEL명현연장（P＜0．05），C조대서제5천화제6천 AEL교B조상응시점명현축단（P＜0．05）；B조화D조해마존재HMGB1대량포장、포외석방정황（P＜0．05），이C조해마구HMGB1포장、포외석방무명현차이（P＞0.05）。4조대서해마총HMGB1표체수평비교차이무통계학의의（P＞0．05），단B조화D조해마JNK표체수평증고（P＜0．05）。결론：강황소능구개선AD대서적학습기억능력，기궤제가능여억제HMGB1적포장、포외석방급JNK적표체하조유관。
AIM:To evaluate the effect of curcumin on impaired learning-memory ability and the expression of high mobility group box protein 1 ( HMGB1 ) and c-Jun N-terminal kinase ( JNK ) in a rat model of Alzheimer disease (AD).METHODS:Male Sprague-Dawley rats, weighing 250~270 g, were randomly divided into 4 groups (n=9):blank control group (group A), model group (group B), curcumin treatment group (group C, curcumin injected intraper-itoneally at 100 mg· kg-1· d-1 for 6 consecutive days) and solvent control group (group D).The rats of AD model were induced by injection of ibotenic acid into the nucleus basalis of Meynert ( NBM) bilaterally.All rats were trained in Morris maze to assess the ability of learning and memory .The expression of HMGB1 and JNK in the hippocampus was detected by the methods of immunohistochemistry and Western blotting .RESULTS:Compared with group A , the average escape laten-cy (AEL) in groups B and D were obviously longer (P<0.05), while AEL in group C in the 5th and 6th days were signif-icantly shorter (P<0.05).The releases of HMGB1 in the CA1 and CA3 areas in groups B and D from the nucleus were a-bundant.Compared with groups B and D , HMGB1 in hippocampal CA1 and CA3 areas in group C secreted out of the nu-cleus decreased obviously (P<0.05).No significant difference of the release of HMGB1 between group A and group C was observed (P>0.05).No significant difference in the expression of HMGB1 in the hippocampus among the 4 groups was found (P>0.05).However, compared with groups B and D , the expression of JNK in group C was decreased obvi-ously (P<0.05).CONCLUSION: Curcumin significantly improves the learning and memory ability of AD rats .The probable mechanisms may be related to inhibiting the release of HMGB 1 from the nucleus of hippocampal neurons and de-creasing the expression of JNK in the hippocampus .
