CAJ | 학술논문

目的：探索P2X7受体在米诺环素（Mino）抑制脂多糖（LPS）刺激的BV-2细胞活化中的作用。方法：将体外培养的BV-2细胞分为5组：空白对照组、LPS处理组、LPS＋0．1μmol／L Mino组、LPS＋1μmol／L Mino组和LPS＋10μmol／L Mino组。分组处理8 h后行real-time PCR检测P2X7受体mRNA表达；处理24 h后观察各组细胞形态学变化，Western blotting检测P2X7受体蛋白表达，取细胞培养液上清行ELISA检测TNF-α和IL-1β的分泌情况。结果：经LPS处理后，BV-2细胞P2X7受体mRNA及蛋白的表达均升高，细胞培养液上清的TNF-α和IL-1β表达升高，同时伴有形态学的改变，而0．1～10μmol／L Mino 能抑制这一趋势，差异有统计学意义（ P ＜0.01）。结论：Mino抑制BV-2细胞活化的机制可能与抑制P2X7受体的活性相关。
목적：탐색P2X7수체재미낙배소（Mino）억제지다당（LPS）자격적BV-2세포활화중적작용。방법：장체외배양적BV-2세포분위5조：공백대조조、LPS처리조、LPS＋0．1μmol／L Mino조、LPS＋1μmol／L Mino조화LPS＋10μmol／L Mino조。분조처리8 h후행real-time PCR검측P2X7수체mRNA표체；처리24 h후관찰각조세포형태학변화，Western blotting검측P2X7수체단백표체，취세포배양액상청행ELISA검측TNF-α화IL-1β적분비정황。결과：경LPS처리후，BV-2세포P2X7수체mRNA급단백적표체균승고，세포배양액상청적TNF-α화IL-1β표체승고，동시반유형태학적개변，이0．1～10μmol／L Mino 능억제저일추세，차이유통계학의의（ P ＜0.01）。결론：Mino억제BV-2세포활화적궤제가능여억제P2X7수체적활성상관。
AIM: To explore the role of P2X7 receptor in inhibition of lipopolysaccharide (LPS)-stimulated BV-2 cell activation by minocycline .METHODS:BV-2 cells were divided into 5 groups:control group, LPS group, LPS+0.1 μmol/L Mino group, LPS+1 μmol/L Mino group and LPS+10 μmol/L Mino group.The expression of P2X7 re-ceptor was determined by real-time PCR and Western blotting .The levels of TNF-αand IL-1βin the microglia culture su-pernatants were measured by ELISA .The morphological changes of the cells were also observed .RESULTS: After ex-posed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels .The concentrations of TNF-αand IL-1βin the microglia culture supernatants also increased .Meanwhile, 0.1~10μmol/L minocycline inhibited those changes in a dose-dependent manner .CONCLUSION:Minocycline inhibits the activation of microglia .The mecha-nism may be related to the P2X7 receptor.