CAJ | 학술논문

目的：探讨Toll样受体（TLR）2和TLR4在牛型结核分枝杆菌卡介苗（BCG）诱导的人肾小管上皮细胞（ HK-2细胞）损伤中的作用。方法：用BCG刺激HK-2细胞，通过荧光定量PCR和Western blotting 方法分别检测TLR2、TLR4、转化生长因子β1（ TGF-β1）和趋化因子CX3CL1的表达。将TLR2抗体和TLR4拮抗剂CLI-90分别与BCG共刺激HK-2细胞，通过荧光定量PCR和Western blotting方法分别检测TGF-β1和CX3CL1的表达水平。结果：BCG刺激HK-2细胞可诱导TLR2、TLR4、TGF-β1和CX3CL1表达上调，TLR2抗体和TLR4拮抗剂可部分抑制BCG引起的TGF-β1和CX3CL1表达上调。结论：BCG可通过激活TLR2和TLR4引起TGF-β1和CX3CL1表达上调，提示TLR2和TLR4介导的信号通路在结核分枝杆菌致肾小管上皮细胞损伤中发挥重要作用。
목적：탐토Toll양수체（TLR）2화TLR4재우형결핵분지간균잡개묘（BCG）유도적인신소관상피세포（ HK-2세포）손상중적작용。방법：용BCG자격HK-2세포，통과형광정량PCR화Western blotting 방법분별검측TLR2、TLR4、전화생장인자β1（ TGF-β1）화추화인자CX3CL1적표체。장TLR2항체화TLR4길항제CLI-90분별여BCG공자격HK-2세포，통과형광정량PCR화Western blotting방법분별검측TGF-β1화CX3CL1적표체수평。결과：BCG자격HK-2세포가유도TLR2、TLR4、TGF-β1화CX3CL1표체상조，TLR2항체화TLR4길항제가부분억제BCG인기적TGF-β1화CX3CL1표체상조。결론：BCG가통과격활TLR2화TLR4인기TGF-β1화CX3CL1표체상조，제시TLR2화TLR4개도적신호통로재결핵분지간균치신소관상피세포손상중발휘중요작용。
AIM:To explore the effect of Toll-like receptor ( TLR) 2 and TLR4 in Mycobacterium bovis Bacil-lus Calmette-Guérin (BCG)-induced human proximal renal tubule epithelial cell (HK-2) injury.METHODS:HK-2 cells were stimulated by BCG, and the expression of TLR2, TLR4, chemokine (C-X3-C motif) ligand 1 (CX3CL1) and trans-forming growth factor beta 1 ( TGF-β1 ) was detected by quantitative real-time PCR and Western blotting .TLR2 monoclonal antibody and TLR4 inhibitor were used to treat the HK-2 cells 1 h before BCG stimulation.The expression of CX3CL1 and TGF-β1 was evaluated by quantitative real-time PCR and Western blotting .RESULTS: BCG increased the expression of TLR2, TLR4, CX3CL1 and TGF-β1 in the HK-2 cells.Additionally, the expression of CX3CL1 and TGF-β1 was inhibited partly by TLR2 monoclonal antibody or TLR4 inhibitor.CONCLUSION:BCG is able to increase the production of TLR 2, TLR4, CX3CL1 and TGF-β1 in the HK-2 cells.TLR2 and TLR4 signaling pathways play important roles in tubule epitheli-al cell injury induced by BCG .