CAJ | 학술논문

目的：研究高交感活性诱发大鼠心肌损伤的氧化应激受体调控机制。方法：Sprague-Dawley （ SD）大鼠随机分为对照组、模型组、普萘洛尔（Pro）组、哌唑嗪（Praz）组、普萘洛尔＋哌唑嗪（Pro＋Praz）组、维生素E （VE）组及普萘洛尔＋哌唑嗪＋维生素E （Pro＋Praz＋VE）组，除对照组外其余各组均腹腔注射去甲肾上腺素（ NE）复制高交感活性引起的心肌损伤模型，同时灌胃给予相应药物，连续给药16 d，期间监测各组动物体重的变化。16 d后进行心室重构指标（心指数和羟脯氨酸含量）、病理组织学检查、氧化／抗氧化指标（ MDA、SOD、CAT、GSH-Px和T-AOC）和能量代谢指标（ Na＋-K＋ATPase和Ca2＋-Mg2＋ATPase）分析。结果：从第9天开始，模型组动物体重与对照组的比较差异有统计学意义（P＜0．05），心指数和左心室肥厚明显增加，氧化／抗氧化和能量代谢障碍；Pro、Praz、Pro＋Praz和VE各组均出现不同程度的动物体重、心指数、左心室肥厚和氧化／抗氧化失衡的改善；Pro、Praz和Pro＋Praz能明显升高左心室Na＋-K＋ATPase和Ca2＋-Mg2＋ATPase的活性，Pro＋Praz作用最明显（ P＜0．05）。结论：肾上腺素受体依赖是高交感活性诱导心肌氧化应激损伤的重要途径。
목적：연구고교감활성유발대서심기손상적양화응격수체조공궤제。방법：Sprague-Dawley （ SD）대서수궤분위대조조、모형조、보내락이（Pro）조、고서진（Praz）조、보내락이＋고서진（Pro＋Praz）조、유생소E （VE）조급보내락이＋고서진＋유생소E （Pro＋Praz＋VE）조，제대조조외기여각조균복강주사거갑신상선소（ NE）복제고교감활성인기적심기손상모형，동시관위급여상응약물，련속급약16 d，기간감측각조동물체중적변화。16 d후진행심실중구지표（심지수화간포안산함량）、병리조직학검사、양화／항양화지표（ MDA、SOD、CAT、GSH-Px화T-AOC）화능량대사지표（ Na＋-K＋ATPase화Ca2＋-Mg2＋ATPase）분석。결과：종제9천개시，모형조동물체중여대조조적비교차이유통계학의의（P＜0．05），심지수화좌심실비후명현증가，양화／항양화화능량대사장애；Pro、Praz、Pro＋Praz화VE각조균출현불동정도적동물체중、심지수、좌심실비후화양화／항양화실형적개선；Pro、Praz화Pro＋Praz능명현승고좌심실Na＋-K＋ATPase화Ca2＋-Mg2＋ATPase적활성，Pro＋Praz작용최명현（ P＜0．05）。결론：신상선소수체의뢰시고교감활성유도심기양화응격손상적중요도경。
AIM:To investigate the dependence of the adrenoceptor regulation on oxidative stress in the rats with cardiac injury induced by high sympathetic activity .METHODS: Healthy SD rats were randomly divided into 7 groups:control, model, propranolol (Pro), prazosin (Praz), Pro+Praz, vitamin E (VE) and Pro+Praz+VE.The rats were intraperitoneally injected with norepinephrine ( NE) for continuous 16 d to reproduce cardiac injury , and treated with the respective drugs .During the experimental process , the body weight was recorded .At the end of the experiments , the following parameters were measured:the ventricular remodeling indexes ( cardiac index and hydroxyproline of the left ven-tricle), histopathologic examination , oxidative/antioxidative indexes [MDA, SOD, catalase (CAT), GSH-Px and total an-tioxidant capacity (T-AOC)], and energy metabolism (Na+-K+ATPase and Ca2+-Mg2+ATPase).RESULTS: The in-crease of body weight in model group was significantly slower than that in control group after 9 d of treatment (P<0.05). The cardiac index and left ventricular hypertrophy were significantly increased .Oxidation/antioxidation and energy metabo-lism were disturbed.In Pro, Praz, Pro+Praz and VE groups, the body weight, cardiac index, left ventricular fibrosis and oxidative/antioxidative dysfunction were ameliorated .Pro, Praz and Pro +Praz increased the activity of Na +-K+ATPase and Ca2+-Mg2+ATPase.Treatment with Pro+Praz showed the best result in all of the indexes (P<0.05).CONCLU-SION:The dependence of adrenoceptor regulation plays an important role in the formation of oxidative stress in the process of rat cardiac injury induced by high sympathetic activity .