CAJ | 학술논문

目的：检测和探讨相同遗传背景结直肠腺瘤-癌组织序列中C／EBP同源蛋白（ CHOP）表达差异及其与细胞增殖／凋亡比率（ PAR）之间的关系。方法：收集23例同时具有3种病理组织学类型的结直肠肿瘤（伴低级别上皮内瘤变腺瘤、伴高级别上皮内瘤变腺瘤和腺癌）病人的手术切除标本及对应正常肠黏膜，采用免疫组织化学SABC法检测CHOP表达水平及细胞增殖指数（PI，即Ki-67阳性细胞率）；采用TUNEL法检测该组织标本中的细胞凋亡指数（AI）。结果：（1）相同遗传背景条件下，CHOP在结直肠腺癌组织中的阳性细胞率显著高于伴高级别上皮内瘤变腺瘤、伴低级别上皮内瘤变腺瘤和正常肠黏膜（P＜0．01）；伴高级别上皮内瘤变腺瘤中的阳性细胞率显著高于伴低级别上皮内瘤变腺瘤和正常肠黏膜（P＜0．01），伴低级别上皮内瘤变腺瘤中的阳性细胞率显著高于正常肠黏膜（P＜0．01）；（2）相同遗传背景条件下，PAR在结直肠腺癌组织显著高于伴高级别上皮内瘤变腺瘤（P＜0．01）、伴低级别上皮内瘤变腺瘤和正常肠黏膜（ P＜0．01）；伴高级别上皮内瘤变腺瘤显著高于伴低级别上皮内瘤变腺瘤和正常肠黏膜（P＜0．01），伴低级别上皮内瘤变腺瘤显著高于正常肠黏膜（P＜0．01）；（3）相同遗传背景条件下， CHOP蛋白表达水平和PAR在伴低级别上皮内瘤变腺瘤、伴高级别上皮内瘤变腺瘤和腺癌组织中呈正相关关系（P＜0．01）。结论：本实验研究了相同遗传背景下结直肠腺瘤-癌组织序列中CHOP表达水平、PAR以及两者之间的关系；随着结直肠腺瘤癌变进展，CHOP表达水平及PAR逐渐升高，且两者呈正相关关系。 CHOP和细胞增殖及凋亡异常可能共同参与结直肠腺瘤癌变过程。
목적：검측화탐토상동유전배경결직장선류-암조직서렬중C／EBP동원단백（ CHOP）표체차이급기여세포증식／조망비솔（ PAR）지간적관계。방법：수집23례동시구유3충병리조직학류형적결직장종류（반저급별상피내류변선류、반고급별상피내류변선류화선암）병인적수술절제표본급대응정상장점막，채용면역조직화학SABC법검측CHOP표체수평급세포증식지수（PI，즉Ki-67양성세포솔）；채용TUNEL법검측해조직표본중적세포조망지수（AI）。결과：（1）상동유전배경조건하，CHOP재결직장선암조직중적양성세포솔현저고우반고급별상피내류변선류、반저급별상피내류변선류화정상장점막（P＜0．01）；반고급별상피내류변선류중적양성세포솔현저고우반저급별상피내류변선류화정상장점막（P＜0．01），반저급별상피내류변선류중적양성세포솔현저고우정상장점막（P＜0．01）；（2）상동유전배경조건하，PAR재결직장선암조직현저고우반고급별상피내류변선류（P＜0．01）、반저급별상피내류변선류화정상장점막（ P＜0．01）；반고급별상피내류변선류현저고우반저급별상피내류변선류화정상장점막（P＜0．01），반저급별상피내류변선류현저고우정상장점막（P＜0．01）；（3）상동유전배경조건하， CHOP단백표체수평화PAR재반저급별상피내류변선류、반고급별상피내류변선류화선암조직중정정상관관계（P＜0．01）。결론：본실험연구료상동유전배경하결직장선류-암조직서렬중CHOP표체수평、PAR이급량자지간적관계；수착결직장선류암변진전，CHOP표체수평급PAR축점승고，차량자정정상관관계。 CHOP화세포증식급조망이상가능공동삼여결직장선류암변과정。
AIM:To investigate the expression of C/EBP homologous protein (CHOP) and its correlation with proliferative/apoptotic ratio (PAR) in colorectal adenoma-carcinoma sequence under the same genetic background .ME-THODS:Four kinds of tissue samples under the same genetic background from 23 patients, including normal colorectal tissue, adenoma with low-grade intraepithelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma samples , were collected .TUNEL method and Ki-67 immunohistochemistry were applied to determine the PAR.The expression of CHOP was detected by immunohistochemistry SABC method .RESULTS: ( 1 ) Under the same genetic background , the level of CHOP expression is significantly higher in colorectal adenocarcinoma than that in the ade -noma with high-grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal muco-sa.The level of CHOP expression was significantly higher in the adenoma with high -grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .The level of CHOP expression was signifi-cantly higher in the adenoma with low-grade intraepithelial neoplasia than that in normal mucosa .(2) Under the same ge-netic background , PAR was significantly higher in the colorectal adenocarcinoma than that in the adenoma with high -grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significant-ly higher in the adenoma with high-grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significantly higher in the adenoma with low-grade intraepithelial neoplasia than that in the normal mucosa.(3) CHOP levels were positively correlated with PAR in the adenoma with low-grade intraepi-thelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma .CONCLUSION:CHOP expression and PAR continuously increased and positively correlated along the adenoma -carcinoma sequence , indica-ting that endoplasmic reticulum stress mediates the carcinogenesis of colorectal adenomas .