CAJ | 학술논문

目的：研究氯喹对地塞米松抑制急性淋巴细胞白血病细胞增殖的促进作用及其机制。方法：采用CCK-8实验观察氯喹对地塞米松抑制人急性淋巴细胞白血病细胞株CEM-C1增殖的促进作用，并采用Western blot-ting、实时定量PCR和LysoTracker Red溶酶体染色法验证此种促进作用的机制。结果：氯喹与地塞米松合用对CEM-C1细胞增殖的抑制明显高于正常对照组（P＜0．01）。氯喹与地塞米松联用上调糖皮质激素受体（GR）的蛋白质水平并抑制溶酶体功能，而溶酶体抑制剂bafilomycin A1亦可上调GR的蛋白质水平。结论：氯喹与地塞米松联用通过溶酶体介导的机制发挥抑制急性淋巴细胞白血病细胞增殖的作用。
목적：연구록규대지새미송억제급성림파세포백혈병세포증식적촉진작용급기궤제。방법：채용CCK-8실험관찰록규대지새미송억제인급성림파세포백혈병세포주CEM-C1증식적촉진작용，병채용Western blot-ting、실시정량PCR화LysoTracker Red용매체염색법험증차충촉진작용적궤제。결과：록규여지새미송합용대CEM-C1세포증식적억제명현고우정상대조조（P＜0．01）。록규여지새미송련용상조당피질격소수체（GR）적단백질수평병억제용매체공능，이용매체억제제bafilomycin A1역가상조GR적단백질수평。결론：록규여지새미송련용통과용매체개도적궤제발휘억제급성림파세포백혈병세포증식적작용。
AIM:To explore the promoting action of chloroquine on the anti-proliferation effect of dexametha-sone on acute lymphoblastic leukemia cells .METHODS:CCK-8 assay was used to assess the viability of the dexametha-sone-resistant human acute lymphoblastic leukemia CEM-C1 cell line treated with the combination of chloroquine and dexa-methasone .Western blotting , quantitative real-time PCR and LysoTracker Red staining were utilized to examine the mecha-nism.RESULTS:Combination of chloroquine and dexamethasone significantly inhibited the proliferation of CEM -C1 cells compared with control group (P<0.01).The combination of chloroquine and dexamethasone increased the abundance of glucocorticoid receptor and inhibited lysosomal function , while lysosomal inhibitor bafilomycin A 1 also increased glucocorti-coid signaling .CONCLUSION:Dexamethasone combined with chloroquine triggers an anti-proliferation effect on CEM-C1 cells via a lysosome-mediated pathway .