CAJ | 학술논문

目的：研究黄连解毒汤（HLT）对丙戊酸钠（SVA）在氯化锂-匹罗卡品致癫痫大鼠体内减毒增效作用。方法采用腹腔注射氯化锂-匹罗卡品制备癫痫大鼠模型。比较未用药、单用SVA及联合用药对大鼠行为学，血清谷丙转氨酶（ALT ）、谷草转氨酶（AST），谷胱甘肽过氧化酶（GSH-Px）活性和丙二醛（MDA）水平以及肝脏组织学变化的影响，同时监测SVA血药浓度。结果 SVA联合 HLT能减少大鼠的癫痫行为，拮抗癫痫大鼠服用SVA导致的ALT、AST 活性升高（P＜0．01）、GSH-Px活性下降（P＜0．01）和肝组织病理改变，并同时降低SVA血药浓度（P＜0．01）。结论 HLT能增强SVA的抗癫痫作用，并降低其肝毒性。
목적：연구황련해독탕（HLT）대병무산납（SVA）재록화리-필라잡품치전간대서체내감독증효작용。방법채용복강주사록화리-필라잡품제비전간대서모형。비교미용약、단용SVA급연합용약대대서행위학，혈청곡병전안매（ALT ）、곡초전안매（AST），곡광감태과양화매（GSH-Px）활성화병이철（MDA）수평이급간장조직학변화적영향，동시감측SVA혈약농도。결과 SVA연합 HLT능감소대서적전간행위，길항전간대서복용SVA도치적ALT、AST 활성승고（P＜0．01）、GSH-Px활성하강（P＜0．01）화간조직병리개변，병동시강저SVA혈약농도（P＜0．01）。결론 HLT능증강SVA적항전간작용，병강저기간독성。
Objective To investigate synergistic effect of Huang Lian Jie Du decoction (HLT) on sodium valproate(SVA) in epi-leptic rats by epileptic rats induced by lithium chloride-pilocarpine .Methods Epileptic rats were induced by intraperitoneal injection of lithium chloride and pilocarpine .The synergistic anti-epilepsy effect of HLT and SVA was investigated .The level of ALT ,AST , SVA in serum ,GSH-Px and MDA were determined at the end of a 4 week adminstration .Then the ultrastructure alteration of the liver were also observed by transmission electon microscope .Results The adminstration of SVA together with HLT can reduce be-havior of epileptic rats .Significant decrease in the level of ALT ,AST ,and SVA in serum of rats and noticeable increases in the ac-tivity of GSH-Px were observed in the co-administered groups compared with administere SVA alone group(P<0 .01) .At the same time ,light microscopic study indicates rats in co-administered SVA and high-dose HLT group has intacthepatic histology .Conclu-sion HLT could has synergistic anti-epileptic effect and relieve the hepatotoxoicity of SVA when co-administered .