CAJ | 학술논문

目的：观察万汶对高原失血性休克兔全血超氧化物歧化酶（SOD）活性及血浆丙二醛（MDA ）浓度的影响。方法将28只日本大耳白兔分为对照组（未进行输液治疗）、生理盐水组、高渗盐水组和万汶组，每组7只。在失血性休克30 min后分别输入4 mL/kg生理盐水、7．5％高渗盐水和万汶（6％羟乙基淀粉，130．0/0．4）进行治疗，检测各组休克前、休克后30 min、输液治疗后30 min全血SOD活性及血浆MDA浓度，并观察平均动脉压（MAP）的变化。结果失血性休克后30 min各组SOD活性均明显降低、MDA浓度均显升高（P＜0．01）。输液治疗后30 min生理盐水组、高渗盐水组SOD及MDA均稳定在失血后30 min水平，组内两时点比较差异无统计学意义（P＞0．05）；万汶组输注30 min后SOD活性及MDA浓度恢复至休克前水平，与失血前比较，差异无统计学意义（P＞0．05），组间同时间点比较差异有统计学意义（P＜0．01）；万汶组MAP在治疗后30 min与休克前比较差异无统计学意义（P＞0．05）。结论高原失血性休克兔输注万汶可以显著提高MAP维持循环稳定并能恢复SOD活性降低氧自由基对机体造成的损伤。
목적：관찰만문대고원실혈성휴극토전혈초양화물기화매（SOD）활성급혈장병이철（MDA ）농도적영향。방법장28지일본대이백토분위대조조（미진행수액치료）、생리염수조、고삼염수조화만문조，매조7지。재실혈성휴극30 min후분별수입4 mL/kg생리염수、7．5％고삼염수화만문（6％간을기정분，130．0/0．4）진행치료，검측각조휴극전、휴극후30 min、수액치료후30 min전혈SOD활성급혈장MDA농도，병관찰평균동맥압（MAP）적변화。결과실혈성휴극후30 min각조SOD활성균명현강저、MDA농도균현승고（P＜0．01）。수액치료후30 min생리염수조、고삼염수조SOD급MDA균은정재실혈후30 min수평，조내량시점비교차이무통계학의의（P＞0．05）；만문조수주30 min후SOD활성급MDA농도회복지휴극전수평，여실혈전비교，차이무통계학의의（P＞0．05），조간동시간점비교차이유통계학의의（P＜0．01）；만문조MAP재치료후30 min여휴극전비교차이무통계학의의（P＞0．05）。결론고원실혈성휴극토수주만문가이현저제고MAP유지순배은정병능회복SOD활성강저양자유기대궤체조성적손상。
Objective To dynamically observe the changes of the plasma superoxide dismutase(SOD) activity and malondialde-hyde(MDA) content of rabbits with hemorrhagic shock in high latitude .Methods 28 rabbits were randomly divided into 4 groups :0 .9% NaCl group ,7 .5% NaCl group ,Voluven(6% HES 130/0 .4) group and control group .The plasma SOD activity and MDA content were measured before shock .At 30 minutes of shock and 30 minutes later of volumetric resuscitation .Then the changes of MAP were observed .Results There were no static difference among 4 groups in the levels of SOD and MDA before shock ;at 30 minutes of shock ,the SOD activity was reduced signicantly and the MDA content was increased in each group (P<0 .01) .Afer fluid infusion there were no obvious change in the plasma levels of SOD and MDA compared with those of shock in 0 .9% NaCl group and in 7 .5% NaCl group(P>0 .05) ,whereas in Voluven group ,compared with those in 0 .9% NaCl and 7 .5% NaCl group the SOD activity was elevated signicantly and the MDA content was decreased (P<0 .01) .Conclusion Voluven can be used in scan-venging oxygen free redicals by recovering the plasma SOD activity in rabbits with hemorrhagic shock in high latitude .