CAJ | 학술논문

目的：探讨负载miRNA-138（miR-138）的重组复制缺陷型腺病毒对人胃癌BGC-823细胞株增殖能力的影响及可能的机制。方法将负载miR-138的重组腺病毒感染人胃癌BGC-823细胞株，采用荧光实时定量PCR检测miR-138表达丰度；采用CCK8方法检测细胞生长曲线；采用Transwell方法检测细胞侵袭能力。结果与Ad-LacZ对照组相比，感染Ad-miR138后，BGC-823细胞内miR-138表达丰度明显升高，差异有统计学意义（1．07±0．07vs．4．89±0．45，P＜0．05）；第6天吸光度明显下降，差异有统计学意义（0．52±0．06vs．0．77±0．06，P＜0．05）；细胞侵袭能力亦显著下降，差异有统计学意义（32．00±11．00vs．56．00±12．00，P＜0．05）。结论miR-138体外可显著抑制人胃癌BGC-823细胞增殖及侵袭能力。
목적：탐토부재miRNA-138（miR-138）적중조복제결함형선병독대인위암BGC-823세포주증식능력적영향급가능적궤제。방법장부재miR-138적중조선병독감염인위암BGC-823세포주，채용형광실시정량PCR검측miR-138표체봉도；채용CCK8방법검측세포생장곡선；채용Transwell방법검측세포침습능력。결과여Ad-LacZ대조조상비，감염Ad-miR138후，BGC-823세포내miR-138표체봉도명현승고，차이유통계학의의（1．07±0．07vs．4．89±0．45，P＜0．05）；제6천흡광도명현하강，차이유통계학의의（0．52±0．06vs．0．77±0．06，P＜0．05）；세포침습능력역현저하강，차이유통계학의의（32．00±11．00vs．56．00±12．00，P＜0．05）。결론miR-138체외가현저억제인위암BGC-823세포증식급침습능력。
Objective To investigate the effect of the replication-deficient adenovirus carrying miR-138(Ad-miR138) on the pro-liferation of human gastric cancer cell line BGC-823 and the possible mechanism .Methods The human gastric cancer cell line BGC-823 was infected with Ad-miR138 .Then the expression level of miR-138 was measured by RT-PCR .The growth curve of BGC-823 was measured using CCK-8 method .The ability of cell invasion was measured using Transwell chambers .Results After infected with Ad-miR138 ,the expression of miR-138 in BGC-823 cells was up -regulated significantly (1 .07 ± 0 .07 vs .4 .89 ± 0 .45 ,P<0 .05) .Absorbance of the 6th day decreased significantly (0 .52 ± 0 .06 vs .0 .77 ± 0 .06 ,P<0 .05) ,and the invasion ability was de-creased obviously (32 .00 ± 11 .00 vs .56 .00 ± 12 .00 ,P<0 .05) .Conclusion miR-138 can effectively suppress the proliferation and invasion of human gastric cancer cell line BGC-823 in vitro .