CAJ | 학술논문

目的：探讨对瞬时受体电位香草酸受体1（TRPV1）及转化生长因子β2（TGF-β2）的检测在儿童UACS中的诊断价值。方法选择32例于我院行腺样体手术的腺样体肥大患儿，其中上气道咳嗽综合征（UACS）组16例，对照组16例。所有患儿均于术前采血进行ELISA检测，术中取腺样体标本进行免疫组化试验。结果 UACS组与对照组相比，用ELISA法和免疫组化法进行检测，TRPV1及TGF-β2的检测值为（14.84±4.38）pg/ml 和（159.02±2.17） pg/ml，较对照组[（4.90±5.64）pg/ml和（101.49±1.70）pg/ml]明显升高，差异有统计学意义（P<0.05）。 ELISA与免疫组化检测结果之间无统计学意义的相关性。免疫组化的敏感性较高，阴性预测值较高，ELISA的特异性较高。结论 TR-PV1及TGF-β2的ELISA及免疫组化检测对UACS有辅助诊断价值。此两项诊断试验具有一定程度的互补性。
목적：탐토대순시수체전위향초산수체1（TRPV1）급전화생장인자β2（TGF-β2）적검측재인동UACS중적진단개치。방법선택32례우아원행선양체수술적선양체비대환인，기중상기도해수종합정（UACS）조16례，대조조16례。소유환인균우술전채혈진행ELISA검측，술중취선양체표본진행면역조화시험。결과 UACS조여대조조상비，용ELISA법화면역조화법진행검측，TRPV1급TGF-β2적검측치위（14.84±4.38）pg/ml 화（159.02±2.17） pg/ml，교대조조[（4.90±5.64）pg/ml화（101.49±1.70）pg/ml]명현승고，차이유통계학의의（P<0.05）。 ELISA여면역조화검측결과지간무통계학의의적상관성。면역조화적민감성교고，음성예측치교고，ELISA적특이성교고。결론 TR-PV1급TGF-β2적ELISA급면역조화검측대UACS유보조진단개치。차량항진단시험구유일정정도적호보성。
Objective The aim of the study was to investigate upper airway cough syndrome (UACS) in children and to determine alternative methods to explore the relationships among transient receptor potential vanniloid 1 (TRPV1) and transforming growth factor-β2 (TGF-β2) and UACS. Methods Thirty-two children with adenoid hypertrophy were included in the study. All children were tested for TRPV1 and TGF-β2 with Enzyme-linked immunosorbent assay(ELISA) and immunohistochemical (IHC)studies. Results The levels of TRPV1 and TGF-β2 were significantly increased in the UACS cases. There was no statistical correlation between ELISA and IHC testing. IHC had higher sensitivity and lower negative likelihood ratio, while ELISA had higher specificity. Conclusion The detections of TRPV1 and TGF-β2 from serum and adenoid body specimens are valuable for the auxiliary diagnosis of UACS. The two diagnostic tests are partially complementary.