CAJ | 학술논문

采用点滴法,在室内测定了蜡蚧轮枝菌 Verticillium lecanii ( Zimm) KMZW-1菌株3.0×104、3.0×105、3.0×106、3.0×107、3.0×108个/mL 5个浓度的分生孢子液对扶桑绵粉蚧(Phenacoccus solenopsis Tins-ley)若虫和雌成虫的毒力。结果表明：蜡蚧轮枝菌KMZW-1菌株对扶桑绵粉蚧具有较高的毒力,在雌成虫初期接种的最高累计校正死亡率大于其他虫态；随着浓度的增加,校正死亡率逐渐增加,在3.0×108个/mL时达到最大；随着时间和浓度的增加,1龄、2龄、3龄若虫和雌成虫初期接种的致死中浓度分别为1.4868×105、3.6617×103、9.8176×104、2.7960×104个/mL,致死中时间分别为5.21、5.55、5.44、4.87 d。
채용점적법,재실내측정료사개륜지균 Verticillium lecanii ( Zimm) KMZW-1균주3.0×104、3.0×105、3.0×106、3.0×107、3.0×108개/mL 5개농도적분생포자액대부상면분개(Phenacoccus solenopsis Tins-ley)약충화자성충적독력。결과표명：사개륜지균KMZW-1균주대부상면분개구유교고적독력,재자성충초기접충적최고루계교정사망솔대우기타충태；수착농도적증가,교정사망솔축점증가,재3.0×108개/mL시체도최대；수착시간화농도적증가,1령、2령、3령약충화자성충초기접충적치사중농도분별위1.4868×105、3.6617×103、9.8176×104、2.7960×104개/mL,치사중시간분별위5.21、5.55、5.44、4.87 d。
The toxicity bioassay of Verticillium lecanii ( Zimm) KMZW-1 to Phenacoccus solenopsis Tinsley under five different concentrations ranged 3. 0×104, 3. 0×105, 3. 0×106, 3. 0×107, 3. 0×108 spores/mL was studied in laboratory by topical application, when the 1st, 2nd and 3rd larvae and the female adults were vacci-nated respectively. The results indicated that the virulence of V. lecanii KMZW-1 to P. solenopsis was high. The accumulative mortality gradually increased with the increase of the concentration, and is highest at the concentra-tion of 3. 0×108 spores/mL. The accumulative mortality of vaccinated female adults was higher than the vaccinat-ed larvae. The highest median lethal concentration of 1st, 2nd and 3rd and adult in 9th day were 1. 486 8×105, 3. 661 7×103, 9. 817 6×104, 2. 796 0×104 spores/mL respectively, and the median lethal time were 5. 21, 5. 55, 5. 44, 4. 87 d respectively.