CAJ | 학술논문

利用提取的玉米小斑病菌C小种( Helminthosporium maydis race C,HMC)毒素处理同核异质体Mo17-C和Mo17-N叶片及叶片中的原生质体并进行凋亡检测。叶片凋亡检测采用DNA凝胶电泳分析,结果表明：Mo17-C和Mo17-N均出现DNA-ladder,其中HMC毒素质量浓度为250μg/mL时,诱导12 h后Mo17-C出现的DNA-ladder最明显,而Mo17-N在HMC毒素质量浓度升至300μg/mL时,诱导12 h后DNA-ladder才最明显。原生质体的凋亡检测采用Hoechst 33258荧光染色法并统计其凋亡率,结果表明：Mo17-C和Mo17-N均出现细胞凋亡的特征,包括产生凋亡小体、环状染色质和染色质边集的现象,且凋亡率均随毒素浓度的增加和诱导时间的延长而呈上升趋势。但对于相同处理,C细胞质的凋亡率显著高于N细胞质,Mo17-C对HMC毒素的敏感性大于Mo17-N。
이용제취적옥미소반병균C소충( Helminthosporium maydis race C,HMC)독소처리동핵이질체Mo17-C화Mo17-N협편급협편중적원생질체병진행조망검측。협편조망검측채용DNA응효전영분석,결과표명：Mo17-C화Mo17-N균출현DNA-ladder,기중HMC독소질량농도위250μg/mL시,유도12 h후Mo17-C출현적DNA-ladder최명현,이Mo17-N재HMC독소질량농도승지300μg/mL시,유도12 h후DNA-ladder재최명현。원생질체적조망검측채용Hoechst 33258형광염색법병통계기조망솔,결과표명：Mo17-C화Mo17-N균출현세포조망적특정,포괄산생조망소체、배상염색질화염색질변집적현상,차조망솔균수독소농도적증가화유도시간적연장이정상승추세。단대우상동처리,C세포질적조망솔현저고우N세포질,Mo17-C대HMC독소적민감성대우Mo17-N。
Apoptosis in leaves and protoplasts from homokaryon maize Mo17-C and Mo17-N treated by Hel-minthosporium maydis race C ( HMC) toxin were detected by DNA ladder and hoechest 33258 respectively. The DNA ladder result indicates that DNA ladder appeared in both Mo17-C and Mo17-N, and the obvious DNA lad-der in Mo17-C appeared after 12 h induction when the concentration of HMC reached 250 μg/mL, but appeared in Mo17-N when the concentration reached 300 μg/mL. Protoplasts of both Mo17-C and Mo17-N revealed apop-totic features such as apoptotic bodies, ring chromatin or chromatin margination. The apoptosis rate of Mo17-C maize protoplasts were significantly higher than that of Mo17-N in the same treatment, and it increased with the increase of treatment time and concentration. So, it is concluded that Mo17-C was more sensitive than Mo17-N.