CAJ | 학술논문

目的：探讨外周血中性粒细胞表面标志物 CD64的表达在儿童支气管肺炎诊断中的价值。方法选取支气管肺炎住院患儿76例，健康对照儿童22例，采用流式细胞术检测外周血中性粒细胞 CD64的表达，同时检测外周血白细胞（WBC）和 C 反应蛋白（CRP）。分别比较各指标在支气管肺炎组与健康对照组以及支原体感染阳性组与阴性组中的差异；比较3种 CD64指数计算方法的敏感性以及与 CRP 的相关性。结果支气管肺炎组 WBC 及 CRP 检测结果与健康对照组相比无显著性差异（P>0.05）；3种 CD64指数计算方法中 Index1及 Index3明显高于健康对照组（P<0.01），Index2结果高于健康对照组，但无统计学差异（P>0.05）。支原体感染阳性组 CD64 Index1、Index2、Index3与支原体感染阴性组相比均无显著性差异（P>0.05）。 ROC 曲线分析结果显示 CD64 Index3即中性粒细胞与淋巴细胞平均荧光强度比值的诊断敏感性最高,最佳临界值为7.04。结论外周血中性粒细胞与淋巴细胞 CD64平均荧光强度比值测定有助于支气管肺炎感染的鉴别诊断。
목적：탐토외주혈중성립세포표면표지물 CD64적표체재인동지기관폐염진단중적개치。방법선취지기관폐염주원환인76례，건강대조인동22례，채용류식세포술검측외주혈중성립세포 CD64적표체，동시검측외주혈백세포（WBC）화 C 반응단백（CRP）。분별비교각지표재지기관폐염조여건강대조조이급지원체감염양성조여음성조중적차이；비교3충 CD64지수계산방법적민감성이급여 CRP 적상관성。결과지기관폐염조 WBC 급 CRP 검측결과여건강대조조상비무현저성차이（P>0.05）；3충 CD64지수계산방법중 Index1급 Index3명현고우건강대조조（P<0.01），Index2결과고우건강대조조，단무통계학차이（P>0.05）。지원체감염양성조 CD64 Index1、Index2、Index3여지원체감염음성조상비균무현저성차이（P>0.05）。 ROC 곡선분석결과현시 CD64 Index3즉중성립세포여림파세포평균형광강도비치적진단민감성최고,최가림계치위7.04。결론외주혈중성립세포여림파세포 CD64평균형광강도비치측정유조우지기관폐염감염적감별진단。
Objective To investigate the clinical value of the expression on peripheral blood neutrophil surface marker CD64 in the diagnosis of bronchopneumonia in children. Methods 76 children with bronchopneumonia and 22 healthy chil-dren were recruited into the study. The levels of peripheral blood neutrophil CD64 were detected by flow cytometry. Peripheral white blood cells (WBC) and C-reactive protein (CRP) were also detected. The differences of WBC, CRP and three index of CD64 were compared in the bronchopneumonia group and healthy controls, mycoplasma infection positive group and negative group respectively. The sensitivity of three kinds of CD64 index calculation methods and the correlation with CRP were analysed. Results The WBC and CRP in bronchial pneumonia group had no significant difference compared with healthy control group (P>0.05). The index1 and index3 of CD64 in bronchial pneumonia group were obviously higher than those of healthy control group, the differences were statistically significant (P<0.01). There were no significant differences of CD64 in mycoplasma in-fection positive group compared with the negative group (P>0.05). ROC curve analysis showed that the diagnostic sensitivity was highest for Index3 which was calculated from the neutrophils and lymphocytes fluorescence ratio, and the cutoff value was 7.04. Conclusion The neutrophils and lymphocytes fluorescence ratio of CD64 contributes to the differential diagnosis of infection in bronchopneumonia.