CAJ | 학술논문

目的：探讨联合检测高尔基体糖蛋白73（GP73）、甲胎蛋白（AFP ）和γ-谷氨酰转移酶同工酶域（GGT-域）对原发性肝癌的诊断意义。方法分别采用化学发光免疫分析法、ELISA 法和聚丙烯酰胺凝胶电泳法检测105例原发性肝细胞癌、60例肝硬化、72例乙型肝炎、30例肝良性肿瘤、90例 HBV 携带者和50例健康体检者血清中 GP73、AFP 和 GGT-域含量，分析 GP73联合 AFP、GGT-域检测对原发性肝癌的诊断价值。结果原发性肝癌组 GP73、AFP 含量及 GGT-域阳性率明显高于良性肝病及正常对照组，差异有统计学意义（P<0.05）；单独检测 GP73、AFP 和 GGT-域对诊断原发性肝癌的特异性分别为75.2％、84.8％和89.1％，敏感性分别为79.0％、57.1％、62.9％，联合检测 GP73、AFP 和 GGT-域可显著提高诊断原发性肝癌的敏感性（97.1％），但特异性有所下降。结论联合检测 GP73、AFP 和 GGT-域作为诊断原发性肝癌的重要指标，可显著提高原发性肝癌的诊断率。
목적：탐토연합검측고이기체당단백73（GP73）、갑태단백（AFP ）화γ-곡안선전이매동공매역（GGT-역）대원발성간암적진단의의。방법분별채용화학발광면역분석법、ELISA 법화취병희선알응효전영법검측105례원발성간세포암、60례간경화、72례을형간염、30례간량성종류、90례 HBV 휴대자화50례건강체검자혈청중 GP73、AFP 화 GGT-역함량，분석 GP73연합 AFP、GGT-역검측대원발성간암적진단개치。결과원발성간암조 GP73、AFP 함량급 GGT-역양성솔명현고우량성간병급정상대조조，차이유통계학의의（P<0.05）；단독검측 GP73、AFP 화 GGT-역대진단원발성간암적특이성분별위75.2％、84.8％화89.1％，민감성분별위79.0％、57.1％、62.9％，연합검측 GP73、AFP 화 GGT-역가현저제고진단원발성간암적민감성（97.1％），단특이성유소하강。결론연합검측 GP73、AFP 화 GGT-역작위진단원발성간암적중요지표，가현저제고원발성간암적진단솔。
Objective To explore the diagnostic value of GP73 combined with AFP,GGT-II detection on primary liver cancer. Methods Serum GP73,AFP,GGT-II levels of 105 cases of primary liver cancer,60 cases of liver cirrhosis,72 cases of hepatitis B,30 cases of benign tumor,90 cases of HBV carriers and 50 cases of healthy controls were detected by ELISA, chemilunescent immunoassay and SDS-PAGE. Analysed the value of GP73 combined with AFP, GGT-II detection on primary liver cancer. Results The levels of GP73,AFP and positive rate of GGT-II were significantly higher than benign liver disease and healthy control group,the differences were statistically significant (P<0.05). Specificity of GP73,AFP and GGT-II in the diagnosis of primary liver cancer was 75.2%,84.8% and 89.1%,respectively. Sensitivity of GP73,AFP and GGT-II in the diag-nosis of primary liver cancer was 79.0%,57.1% and 62.9%,respectively. The joint detection GP73,AFP and GGT-II could im-prove the sensitivity for the diagnosis of primary liver cancer, but the specificity decreased. Conclusion The combined de-tection of GP73,AFP and GGT-II is an important index for the diagnosis of primary liver cancer,which can improve the diag-nosis rate of primary liver cancer.