CAJ | 학술논문

以不同铜离子添加量(0,10,25,40,55和110 mg/kg)的饲料喂养斑节对虾(Penaeus monodon)8周,测定对虾的血细胞总数(THC),并应用流式细胞术测定血细胞中含颗粒细胞(小颗粒细胞和大颗粒细胞)的比例、血细胞凋亡率、活性氧(ROS)含量和酯酶活性。结果显示,当铜添加量为10~55 mg/kg时,与对照组相比,对虾的 THC、血细胞凋亡率、ROS 含量和酯酶活性均没有显著的变化(P>0.05)；铜添加量为25~110 mg/kg时,含颗粒细胞的比例显著提高(P<0.05)；铜添加量为110 mg/kg时, ROS含量显著高于其他组(P<0.05),血细胞凋亡率高于25和55 mg/kg组(P<0.05), THC低于55 mg/kg组(P<0.05),酯酶活性低于25mg/kg组(P<0.05)。这些结果表明,适宜的饲料铜含量有利于两类颗粒细胞的生成；铜添加过量(110 mg/kg)会诱导血细胞ROS的产生,造成氧化损伤,从而导致凋亡率上升和THC下降。综上所述,在本实验日粮条件下,针对斑节对虾的血细胞功能,其饲料中铜适宜添加含量范围为25~55mg/kg。
이불동동리자첨가량(0,10,25,40,55화110 mg/kg)적사료위양반절대하(Penaeus monodon)8주,측정대하적혈세포총수(THC),병응용류식세포술측정혈세포중함과립세포(소과립세포화대과립세포)적비례、혈세포조망솔、활성양(ROS)함량화지매활성。결과현시,당동첨가량위10~55 mg/kg시,여대조조상비,대하적 THC、혈세포조망솔、ROS 함량화지매활성균몰유현저적변화(P>0.05)；동첨가량위25~110 mg/kg시,함과립세포적비례현저제고(P<0.05)；동첨가량위110 mg/kg시, ROS함량현저고우기타조(P<0.05),혈세포조망솔고우25화55 mg/kg조(P<0.05), THC저우55 mg/kg조(P<0.05),지매활성저우25mg/kg조(P<0.05)。저사결과표명,괄의적사료동함량유리우량류과립세포적생성；동첨가과량(110 mg/kg)회유도혈세포ROS적산생,조성양화손상,종이도치조망솔상승화THC하강。종상소술,재본실험일량조건하,침대반절대하적혈세포공능,기사료중동괄의첨가함량범위위25~55mg/kg。
The black tigershrimps (Penaeus monodon) were fed with diets supplemented copper at different con-centrations (0, 10, 25, 40, 55 and 110 mg/kg) for 8-week, and the total haemocyte count (THC), percentage of cells contained granular, apoptotic cell ratio, reactive oxygen species (ROS) production and esterase activity were ana-lyzed. The results showed that THC, apoptotic cell ratio, ROS production and esterase activity were not affected by 10~55 mg/kg supplemental Cu, compared to the control group. Percentage of cells contained granular was signifi-cantly increased when the shrimpswere fed with diets containing 10~55 mg/kg supplemental Cu. In shrimps fed with diets containing 110 mg/kg supplemental Cu, the ROS production in haemocytes was significantly increased, and the apoptotic cell ratio was higher than that of the shrimps fed with diets supplemented with 25 and 55 mg/kg Cu, while THC was lower than that of the shrimps fed with diets supplemented with 55 mg/kg Cu, and the esterase activity was lower than that of the shrimps fed with diets supplemented with 25 mg/kg Cu. These results indicate that adequatedietary Cu concentration could increase the number of cells contained granular. Excess supplemental Cu (110 mg/kg) will induce the production of ROS in haemocytes, and then induce apoptosis and THC decreaseby oxidative damage.In conclusion, the adequate supplemental Cu concentration in diet for haemocyte functions in P. monodon is 25~55 mg/kg.