CAJ | 학술논문

将雄性SD大鼠24只随机分为4组，对照组自由取食普通饲料和水，模型组自由取食高糖高脂饲料和水，这2组每天灌胃灭菌水，绿原酸高、低剂量组自由取食普通饲料和水，且每天按照90 mg/kg，20 mg/kg剂量分别灌胃绿原酸，12周后测肝脏组织中总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）和游离脂肪酸（NEFA）含量，并采用RT-PCR方法检测肝脏SOD与GSH-PX mRNA的表达量。结果表明：与模型组相比，绿原酸低剂量组肝脏中NEFA和MDA显著下降（P<0.01，P<0.05），绿原酸高剂量组肝脏NEFA和MDA显著下降（P<0.01）。与模型组相比，绿原酸低、高剂量组肝脏T-SOD显著上升（P<0.01），高剂量组肝脏CAT指标显著上升（P<0.01）。低、高剂量组SOD与GSH-PX mRNA表达水平高于模型组且呈剂量依赖性，但没有显著性差异。绿原酸提高了高脂高糖饲喂的SD大鼠肝脏抗氧化能力。
장웅성SD대서24지수궤분위4조，대조조자유취식보통사료화수，모형조자유취식고당고지사료화수，저2조매천관위멸균수，록원산고、저제량조자유취식보통사료화수，차매천안조90 mg/kg，20 mg/kg제량분별관위록원산，12주후측간장조직중총초양화물기화매（T-SOD）、과양화경매（CAT）、곡광감태과양화물매（GSH-PX）화유리지방산（NEFA）함량，병채용RT-PCR방법검측간장SOD여GSH-PX mRNA적표체량。결과표명：여모형조상비，록원산저제량조간장중NEFA화MDA현저하강（P<0.01，P<0.05），록원산고제량조간장NEFA화MDA현저하강（P<0.01）。여모형조상비，록원산저、고제량조간장T-SOD현저상승（P<0.01），고제량조간장CAT지표현저상승（P<0.01）。저、고제량조SOD여GSH-PX mRNA표체수평고우모형조차정제량의뢰성，단몰유현저성차이。록원산제고료고지고당사위적SD대서간장항양화능력。
24 male SD mice were divided into four groups randomly. The control group can freely eat normal feedstuff and water and the model group can freely eat high-fat and high-sugar feedstuff, while both the groups perfused sterile water everyday;the group with high dosage of chlorogenic acid and the group with low dosage of chlorogenic acid both can freely eat normal feedstuff and water, while the former one perfused 90 mg/kg of chlorogenic acid everyday and the latter one perfused 20 mg/kg of chlorogenic acid everyday. After 12 weeks, the contents of T-SOD, CAT, GSH-PX and NEFA in liver tissue were measured, and the mRNA expression level of SOD and GSH-PX in liver was detected by RT-PCR. The results showed that in comparison with model group, the contents of NEFA and MDA of liver in group with low dosage of chlorogenic acid decreased significantly (P<0.01 and P<0.05), and those in group with high dosage of chlorogenic acid also decreased significantly (P<0.01);the content of T-SOD of liver in both groups with low or high dosage of chlorogenic acid increased significantly (P<0.01), while the content of CAT of liver in group with high dosage of chlorogenic acid also increased significantly (P<0.01). The mRNA expression level of SOD and GSH-PX of liver in both groups with low or high dosage of chlorogenic acid was higher than those in model group with dose-dependent manner, but there was no significant difference between them. Therefore, the chlorogenic acid enhanced antioxidant capacity of liver for SD mouse fed with high-fat and high-sugar feedstuff.