CAJ | 학술논문

为了建立高效液相色谱测定水产品中四环素类、喹诺酮类抗生素多组分残留的方法，样品经酸化乙腈提取，正己烷脱脂净化，高效液相色谱分离，紫外检测器检测，外标法定量。结果表明：6种抗生素在0.01～2.00μg/mL范围内线性关系良好，最低检出限为2.00～8.00μg/kg。添加水平为10、20、50μg/kg时，回收率达60.4%～87.7%，RSD≤8.2%。该方法简便、准确，可用于水产品中四环素类、喹诺酮类6种抗生素的同时测定。
위료건립고효액상색보측정수산품중사배소류、규낙동류항생소다조분잔류적방법，양품경산화을정제취，정기완탈지정화，고효액상색보분리，자외검측기검측，외표법정량。결과표명：6충항생소재0.01～2.00μg/mL범위내선성관계량호，최저검출한위2.00～8.00μg/kg。첨가수평위10、20、50μg/kg시，회수솔체60.4%～87.7%，RSD≤8.2%。해방법간편、준학，가용우수산품중사배소류、규낙동류6충항생소적동시측정。
In order to establish a HPLC method to determine tetracycline and quinolone antibiotics residues in aquatic products, the samples were extracted by acidified acetonitrile, degreased and purified by n-hexane, and separated by HPLC, detected by UV detector, and then be conducted quantity by external reference method. The results showed that six antibiotics showed a good linear correlation within 0.01~2.00 μg/mL, and the minimum detection limit was 2.00~8.00 μg/kg. When adding level was at 10, 20 and 50 μg/kg, the recovery reached 60.4%~87.7% with RSD≤8.2%. The method is simple and accurate, therefore can be used for simultaneous determining six tetracycline and quinolone antibiotics in aquatic products.