CAJ | 학술논문

目的：研究血清IL-17在结直肠癌患者术前、术后1d及术后7d不同时段中的表达水平及其临床意义。方法应用双抗体夹心ABC-ELISA法检测20例健康志愿者（对照组）和55例结直肠癌患者术前（A组）、术后1d（B组）、术后7d （C组）血清中的IL-17浓度，分析其与结直肠癌临床病理特征的关系和术前、术后不同时段的变化。结果 A组、B组血清IL-17表达水平明显高于对照组（P<0.05），C组血清IL-17浓度高于对照组，但差异无统计学意义（P>0.05）。 C组血清IL-17表达水平明显低于A组与B组（P<0.05），B组血清IL-17浓度高于A组，但差异无统计学意义（P>0.05）。 IL-17的表达水平与肿瘤浸润深度、肿瘤分化程度、TNM分期、有无肿瘤神经束侵犯有关（P<0.05），T4b患者IL-17浓度明显高于T1~3、T4a患者（P<0.05），低分化患者IL-17浓度明显高于中、高分化患者（P<0.05），Ⅲ期患者血清IL-17浓度明显高于Ⅰ~II期患者（P<0.05），有肿瘤神经束侵犯患者IL-17浓度明显高于无侵犯患者（P<0.05）。 IL-17表达水平与患者性别、年龄、肿瘤大小、肿瘤部位及脉管有无侵犯等因素无关（P>0.05）。结论 IL-17在结直肠癌患者血清中高表达，与肿瘤浸润深度、肿瘤分化程度、TNM分期有关，其可能在结直肠癌的发生、发展中起重要的作用。
목적：연구혈청IL-17재결직장암환자술전、술후1d급술후7d불동시단중적표체수평급기림상의의。방법응용쌍항체협심ABC-ELISA법검측20례건강지원자（대조조）화55례결직장암환자술전（A조）、술후1d（B조）、술후7d （C조）혈청중적IL-17농도，분석기여결직장암림상병리특정적관계화술전、술후불동시단적변화。결과 A조、B조혈청IL-17표체수평명현고우대조조（P<0.05），C조혈청IL-17농도고우대조조，단차이무통계학의의（P>0.05）。 C조혈청IL-17표체수평명현저우A조여B조（P<0.05），B조혈청IL-17농도고우A조，단차이무통계학의의（P>0.05）。 IL-17적표체수평여종류침윤심도、종류분화정도、TNM분기、유무종류신경속침범유관（P<0.05），T4b환자IL-17농도명현고우T1~3、T4a환자（P<0.05），저분화환자IL-17농도명현고우중、고분화환자（P<0.05），Ⅲ기환자혈청IL-17농도명현고우Ⅰ~II기환자（P<0.05），유종류신경속침범환자IL-17농도명현고우무침범환자（P<0.05）。 IL-17표체수평여환자성별、년령、종류대소、종류부위급맥관유무침범등인소무관（P>0.05）。결론 IL-17재결직장암환자혈청중고표체，여종류침윤심도、종류분화정도、TNM분기유관，기가능재결직장암적발생、발전중기중요적작용。
Objective To study the expression level and clinical significance of serum IL-17 in colorectal cancer pa-tients at different time:before surgery, 1 day and 7 days after operation. Methods Detected the concentration of serum IL-17 of 20 healthy volunteers (the control group) and 55 cases of colorectal cancer patients at pre-operative (group A), 1 day after operation(group B), 7 days after operation (group C) by double antibody sandwich ABC-ELISA, and analyzed the relationship between IL-17 and clinical pathological characteristics of colorectal cancer , the preoperative and postoperative changes at dif-ferent time. Results The expression level of serum IL-17 of patients in group A and group B was significantly higher than the control group (P<0.05), and the concentration of serum IL-17 of group C was also higher than that of the control group, but there was no statistically significant difference (P>0.05). The expression level of serum IL-17 of group C was obviously lower than that of group A and group B (P<0.05),while the concentration of serum IL-17 of group B was higher than that of group A, which had no statistically significant difference (P>0.05). The expression level of IL-17 was associated with infiltration depth of tumor, differentiation degree of tumor, TNM stages and whether it related to nerve tract invasion or not (P<0.05), and the con-centration of serum IL-17 in T4b patients was significantly higher than T1~3,T4a patients(P<0.05),and it was also significantly higher in the patients with low differentiation than the patients with moderate differentiation and high differentiation (P<0.05), the concentration of serum IL-17 in the patients with stage Ⅲ was higher than that of patients stage Ⅰ~II, and the concentra-tion of patients with tumor nerve bundle invasion was significantly higher than that of patients with no invasion(P<0.05). The ex-pression level of IL-17 had nothing to do with such factors as patient gender, age, tumor size, tumor location, and the presence of vascular infringement(P>0.05). Conclusion IL-17 has a high expression in the serum of colorectal cancer patients,and it is associated with tumor infiltration depth, differentiation degree of tumor, TNM stages,and it may play an important role in the occurrence and development of colorectal cancer.