CAJ | 학술논문

目的：探讨低氧（7.5%O2）和高氧（20%O2）培养体系对人早期胚胎（D1~D3）体外发育及胚胎移植临床结局的影响．方法选取2012年2月至2013年8月在云南省第二人民医院生殖医学中心接受常规IVF-ET治疗，行D3新鲜胚胎移植的周期资料，共280个周期，比较7.5%O2低氧组（120个周期）和20%O2高氧组（160个周期）早期胚胎体外发育的结果和胚胎移植的临床结局．结果低氧组D3优级胚胎形态外观上明显有别于次优级胚胎（即传统优级胚胎），呈卵裂球紧贴，裂球边界渐模糊的形态学特征，且优级胚胎率显著高于高氧组（66.22%与47.54%），但2组受精率、卵裂率和可用胚胎率比较差异无统计学意义．低氧组胚胎的着床率显著高于高氧组（42.94%与31.01%），生化妊娠率（62.22%与47.06%）和临床妊娠率（53.33%与39.22%）也均比高氧组高．结论体外低氧培养系统能产生质量更高的人早期胚胎，对提高IVF临床结局有益．
목적：탐토저양（7.5%O2）화고양（20%O2）배양체계대인조기배태（D1~D3）체외발육급배태이식림상결국적영향．방법선취2012년2월지2013년8월재운남성제이인민의원생식의학중심접수상규IVF-ET치료，행D3신선배태이식적주기자료，공280개주기，비교7.5%O2저양조（120개주기）화20%O2고양조（160개주기）조기배태체외발육적결과화배태이식적림상결국．결과저양조D3우급배태형태외관상명현유별우차우급배태（즉전통우급배태），정란렬구긴첩，렬구변계점모호적형태학특정，차우급배태솔현저고우고양조（66.22%여47.54%），단2조수정솔、란렬솔화가용배태솔비교차이무통계학의의．저양조배태적착상솔현저고우고양조（42.94%여31.01%），생화임신솔（62.22%여47.06%）화림상임신솔（53.33%여39.22%）야균비고양조고．결론체외저양배양계통능산생질량경고적인조기배태，대제고IVF림상결국유익．
Objective To investigate the effect of low oxygen tension (7.5% O2) on human embryo development in vitro and outcome of women undergoing IVF-ET. Methods The data of 280 routine IVF cycles with fresh embryo transfer on day3 conducted in the 2nd People's Hospital of Yunnan Province from February 2012 to August 2013 were retrospectively analyzed. Two groups, 7.5%O2 low oxygen tension (n=120) and 20%O2 high oxygen tension (n=160),were setup for early embryo (D1-D3) development in vitro. Results Good quality embryo on day 3 in low oxygen tension group showed morphologically appearance of blastomere mutually clinging to each other,a characteristic of slight compaction. Higher rate of good quality embryo (66.22%V. 47.54%) and of clinical pregnancy (53.33%v. 39.22%) were found in the low oxygen tension group. There were no significantly differences in rates of fertilization,cleavage and embryo utilization respectively between the two groups ( all>0. 05) . Conclusion High quality early stage human embryos can be developed in vitro in low oxygen tension, and may improve clinic outcome of IVF.