河北中医
河北中醫
하북중의
HEBEI JORNAL OF TRADITIONAL CHINESE MEDICINE
2014年
2期
288-290
,共3页
结肠炎%溃疡性%中药疗法%模型%动物%大鼠
結腸炎%潰瘍性%中藥療法%模型%動物%大鼠
결장염%궤양성%중약요법%모형%동물%대서
Colitis%Ulcerative%Traditional Chinese medicine therapy%Models%Animal%Rats
目的：观察振脾汤对溃疡性结肠炎（ UC）模型大鼠灌胃。方法将48只Wistar大鼠随机分为正常组、模型组、振脾汤组及柳氮磺吡啶片组，每组12只，采用三硝基苯磺酸（ TNBS）/乙醇方法建立UC模型。造模成功2周后，振脾汤组予振脾汤灌胃，柳氮磺胺吡啶片组予柳氮磺吡啶片溶液灌胃，正常组及模型组均予等容积的蒸馏水灌胃。连续灌胃2周后。观察各组大鼠的一般状态、结肠黏膜的大体形态学及组织病理学变化。结果给药前模型组大鼠体质量、结肠黏膜大体形态学损伤评分及结肠黏膜组织病理学损伤评分与正常组同期比较差异均有统计学意义（P＜0．05）；给药1周后，振脾汤组及柳氮磺胺吡啶片组大鼠体质量、结肠黏膜大体形态学损伤评分及结肠黏膜组织病理学损伤评分与模型组同期比较差异均无统计学意义（P＜0．05）；给药2周后，振脾汤组及柳氮磺胺吡啶片组大鼠体质量、结肠黏膜大体形态学损伤评分及结肠黏膜组织病理学损伤评分与模型组同期比较差异均有统计学意义（P＜0．05），但2组间比较差异无统计学意义（P＞0．05）。结论振脾汤可以明显改善UC模型大鼠一般情况，增加体质量，改善微循环，抑制炎症反应，修复溃疡，更适用于UC的维持治疗。
目的：觀察振脾湯對潰瘍性結腸炎（ UC）模型大鼠灌胃。方法將48隻Wistar大鼠隨機分為正常組、模型組、振脾湯組及柳氮磺吡啶片組，每組12隻，採用三硝基苯磺痠（ TNBS）/乙醇方法建立UC模型。造模成功2週後，振脾湯組予振脾湯灌胃，柳氮磺胺吡啶片組予柳氮磺吡啶片溶液灌胃，正常組及模型組均予等容積的蒸餾水灌胃。連續灌胃2週後。觀察各組大鼠的一般狀態、結腸黏膜的大體形態學及組織病理學變化。結果給藥前模型組大鼠體質量、結腸黏膜大體形態學損傷評分及結腸黏膜組織病理學損傷評分與正常組同期比較差異均有統計學意義（P＜0．05）；給藥1週後，振脾湯組及柳氮磺胺吡啶片組大鼠體質量、結腸黏膜大體形態學損傷評分及結腸黏膜組織病理學損傷評分與模型組同期比較差異均無統計學意義（P＜0．05）；給藥2週後，振脾湯組及柳氮磺胺吡啶片組大鼠體質量、結腸黏膜大體形態學損傷評分及結腸黏膜組織病理學損傷評分與模型組同期比較差異均有統計學意義（P＜0．05），但2組間比較差異無統計學意義（P＞0．05）。結論振脾湯可以明顯改善UC模型大鼠一般情況，增加體質量，改善微循環，抑製炎癥反應，脩複潰瘍，更適用于UC的維持治療。
목적：관찰진비탕대궤양성결장염（ UC）모형대서관위。방법장48지Wistar대서수궤분위정상조、모형조、진비탕조급류담광필정편조，매조12지，채용삼초기분광산（ TNBS）/을순방법건립UC모형。조모성공2주후，진비탕조여진비탕관위，류담광알필정편조여류담광필정편용액관위，정상조급모형조균여등용적적증류수관위。련속관위2주후。관찰각조대서적일반상태、결장점막적대체형태학급조직병이학변화。결과급약전모형조대서체질량、결장점막대체형태학손상평분급결장점막조직병이학손상평분여정상조동기비교차이균유통계학의의（P＜0．05）；급약1주후，진비탕조급류담광알필정편조대서체질량、결장점막대체형태학손상평분급결장점막조직병이학손상평분여모형조동기비교차이균무통계학의의（P＜0．05）；급약2주후，진비탕조급류담광알필정편조대서체질량、결장점막대체형태학손상평분급결장점막조직병이학손상평분여모형조동기비교차이균유통계학의의（P＜0．05），단2조간비교차이무통계학의의（P＞0．05）。결론진비탕가이명현개선UC모형대서일반정황，증가체질량，개선미순배，억제염증반응，수복궤양，경괄용우UC적유지치료。
Objective To investigate the effect of Zhenpi decoction on the model rats with ulcerative colitis ( UC) .Methods 48 Wistar rats were randomly divided into normal group , model group , Zhenpi decoction group and Sulfasalazine tablets group , 12 rats in each group.UC model rats were established by Trinitrobenzene sulfonic acid ( TNBS) /ethanol method .At two weeks after successful modelling , rats in Zhenpi decoction group and Sul-fasalazine tablets group separately received intragastric administration of Zhenpi decoction and Sulfasalazine tablets so -lution.Rats in normal group and model group received intragastric administration of isometric distilled water for two weeks .The changes of general stats , gross morphology and histopathology of colonic mucosa of rats in each group were observed.Results There were significant differences on rat BMI , gross morphological injury score and histopatho-logical injury score of colonic mucosa of rats in model group , Zhenpi decoction group and Sulfasalazine tablets group before treatment compared with those in normal group (P<0.05).There was no difference between rats in model group, Zhenpi decoction group and Sulfasalazine tablets group on rat BMI , gross morphological injury score and his-topathological injury score of colonic mucosa of rats after a week of treatment (P>0.05).There were significant difference between rats in model group , Zhenpi decoction group and Sulfasalazine tablets group on rat BMI , gross morphological injury score and histopathological injury score of colonic mucosa of rats after two weeks of treatment ( P<0.05).There was no significant difference between Zhenpi decoction group and Sulfasalazine tablets group (P>0.05).Conclusion The effect of Zhenpi decoction is significant on UC model rats , can significantly improve the general situation in rats , increase rats body mass , improve microcirculation , inhibit inflammation , repair ulcers and pain etc ., it is more suitable for the maintenance treatment of UC .
