CAJ | 학술논문

目的：探讨基质金属蛋白酶抑制剂GM6001在青光眼滤过术后滤过道瘢痕化中的作用。方法随机取青紫兰兔24只，双眼行抗青光眼小梁切除术，术后右眼结膜下注射GM6001作为实验组，左眼注射PBS作为对照组，1次/d，监测眼压，并通过免疫组化方法标记抗增殖细胞核抗原（PCNA）和α-平滑肌肌动蛋白（α-SMA），观察滤过道成纤维细胞增殖情况。结果术后7，14，21，28 d，实验组较对照组眼压低，术后各时间点实验组PCNA和α-SMA阳性细胞数低于对照组，均具有显著性差异(P<0.01)。结论GM6001具有抑制兔眼滤过术后滤过道成纤维细胞过度增殖，减少瘢痕形成和延长功能性滤过泡存在时间的作用。
목적：탐토기질금속단백매억제제GM6001재청광안려과술후려과도반흔화중적작용。방법수궤취청자란토24지，쌍안행항청광안소량절제술，술후우안결막하주사GM6001작위실험조，좌안주사PBS작위대조조，1차/d，감측안압，병통과면역조화방법표기항증식세포핵항원（PCNA）화α-평활기기동단백（α-SMA），관찰려과도성섬유세포증식정황。결과술후7，14，21，28 d，실험조교대조조안압저，술후각시간점실험조PCNA화α-SMA양성세포수저우대조조，균구유현저성차이(P<0.01)。결론GM6001구유억제토안려과술후려과도성섬유세포과도증식，감소반흔형성화연장공능성려과포존재시간적작용。
Objective To study the effect of matrix metalloproteinases inhibitor GM6001 in suppressing scar tissue formation in the filtering passage after glaucoma filtration surgery. Methods Twenty- four pigmented rabbits (48 eyes) underwent trabeculectomy followed by subconjunctival injection of GM6001 in the right eye (treated eyes) and injection of PBS in the left eye (control) once a day. The intraocular pressure was monitored postoperatively and proliferating cell nuclear antigen (PCNA)- and α- smooth muscle actin (α- SMA)- positive cells in the filtering pathway were detected using immunohistochemistry. Results On postoperative days 7, 14, 21, and 28, the intraocular pressure was significantly lower in the treated eyes (GM6001) than in the control eyes (P<0.01). The counts of PCNA-andα-SMA-positive cells were also significantly lowered in the treated than in the control eyes (P<0.01). Conclusion GM6001 can inhibit excessive proliferation of the fibroblasts in the filtering pathway to suppress scar tissue formation and prolong the existence of the functional filtration bleb in rabbits.