CAJ | 학술논문

目的：研究体外闭合环路内自体猪血常温血液持续循环灌注器官96 h并维持较好结构和功能状态的可行性。方法8个猪肝脏分别在体外闭合管路内用自体猪血灌注，均持续96 h。实验中需行切肝、低温保存、静脉置管、常温灌注、血液氧合。通过代谢、合成、血流动力学、组织学参数来评价实验中猪肝的活力。结果经过96 h自体猪血常温血液体外灌注之后，体外肝脏维持了正常生理水平的pH和电解质，肝脏合成功能（补体和V因子）持续存在。血流动力学参数保持在正常生理范围内。组织学检查显示肝脏细微和整体结构保存完好。结论自体猪血体外灌注离体肝脏96 h并维持较好的存活状态是可行的。
목적：연구체외폐합배로내자체저혈상온혈액지속순배관주기관96 h병유지교호결구화공능상태적가행성。방법8개저간장분별재체외폐합관로내용자체저혈관주，균지속96 h。실험중수행절간、저온보존、정맥치관、상온관주、혈액양합。통과대사、합성、혈류동역학、조직학삼수래평개실험중저간적활력。결과경과96 h자체저혈상온혈액체외관주지후，체외간장유지료정상생리수평적pH화전해질，간장합성공능（보체화V인자）지속존재。혈류동역학삼수보지재정상생리범위내。조직학검사현시간장세미화정체결구보존완호。결론자체저혈체외관주리체간장96 h병유지교호적존활상태시가행적。
Objective To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporeal perfusion using autologous blood. Methods Eight extracorporeal porcine liver perfusions using autologous blood were performed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion with normothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, and histologic parameters. Results After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated livers maintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement and factor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histological inspection demonstrated good preservation of the liver with a good architectural integrity. Conclusion It is possible to sustain the viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.