南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2014年
2期
210-213
,共4页
熊兴东%成捷%刘新光%唐韶景%罗喜平
熊興東%成捷%劉新光%唐韶景%囉喜平
웅흥동%성첩%류신광%당소경%라희평
miR-124%单核苷酸多态性%宫颈癌%遗传易感性
miR-124%單覈苷痠多態性%宮頸癌%遺傳易感性
miR-124%단핵감산다태성%궁경암%유전역감성
miR-124%single nucleotide polymorphism%cervical cancer%genetic susceptibility
目的：探讨广东地区汉族妇女miR-124 rs531564基因多态性与宫颈癌遗传易感的关系，为宫颈癌的预防和治疗提供新的思路。方法利用聚合酶链式反应-连接酶检测反应（PCR-LDR）技术，对107例宫颈癌患者和208名健康对照个体的miR-124基因rs531564多态位点进行分型。采用非条件逻辑回归分析统计该多态位点与宫颈癌易感的相关性，计算相对危险度的比值比（OR）及95%置信区间（CI）。结果 HPV感染在病例组中的分布频率显著高于正常对照组（93.1%vs 16.8%，P<0.001），提示HPV感染是广东地区宫颈癌发病的重要危险因素。等位基因的结果显示，miR-124 rs531564 G等位基因在病例组中的分布频率显著低于正常对照组（8.0%vs 15.1%，P=0.014）。与CC基因型相比，携带G等位基因的个体（CG+GG）显著降低患宫颈癌的风险（OR=0.47，95%CI=0.26-0.88，P=0.017）。而且G等位基因降低个体患宫颈癌风险的趋势在年龄≥45岁的人群中更加明显（OR=0.28，95%CI=0.10-0.76，P=0.012）。结论 miR-124基因rs531564基因多态性与广东地区汉族妇女宫颈癌的遗传易感有密切关系，G等位基因可能是降低宫颈癌发病风险的重要因素。
目的：探討廣東地區漢族婦女miR-124 rs531564基因多態性與宮頸癌遺傳易感的關繫，為宮頸癌的預防和治療提供新的思路。方法利用聚閤酶鏈式反應-連接酶檢測反應（PCR-LDR）技術，對107例宮頸癌患者和208名健康對照箇體的miR-124基因rs531564多態位點進行分型。採用非條件邏輯迴歸分析統計該多態位點與宮頸癌易感的相關性，計算相對危險度的比值比（OR）及95%置信區間（CI）。結果 HPV感染在病例組中的分佈頻率顯著高于正常對照組（93.1%vs 16.8%，P<0.001），提示HPV感染是廣東地區宮頸癌髮病的重要危險因素。等位基因的結果顯示，miR-124 rs531564 G等位基因在病例組中的分佈頻率顯著低于正常對照組（8.0%vs 15.1%，P=0.014）。與CC基因型相比，攜帶G等位基因的箇體（CG+GG）顯著降低患宮頸癌的風險（OR=0.47，95%CI=0.26-0.88，P=0.017）。而且G等位基因降低箇體患宮頸癌風險的趨勢在年齡≥45歲的人群中更加明顯（OR=0.28，95%CI=0.10-0.76，P=0.012）。結論 miR-124基因rs531564基因多態性與廣東地區漢族婦女宮頸癌的遺傳易感有密切關繫，G等位基因可能是降低宮頸癌髮病風險的重要因素。
목적：탐토엄동지구한족부녀miR-124 rs531564기인다태성여궁경암유전역감적관계，위궁경암적예방화치료제공신적사로。방법이용취합매련식반응-련접매검측반응（PCR-LDR）기술，대107례궁경암환자화208명건강대조개체적miR-124기인rs531564다태위점진행분형。채용비조건라집회귀분석통계해다태위점여궁경암역감적상관성，계산상대위험도적비치비（OR）급95%치신구간（CI）。결과 HPV감염재병례조중적분포빈솔현저고우정상대조조（93.1%vs 16.8%，P<0.001），제시HPV감염시엄동지구궁경암발병적중요위험인소。등위기인적결과현시，miR-124 rs531564 G등위기인재병례조중적분포빈솔현저저우정상대조조（8.0%vs 15.1%，P=0.014）。여CC기인형상비，휴대G등위기인적개체（CG+GG）현저강저환궁경암적풍험（OR=0.47，95%CI=0.26-0.88，P=0.017）。이차G등위기인강저개체환궁경암풍험적추세재년령≥45세적인군중경가명현（OR=0.28，95%CI=0.10-0.76，P=0.012）。결론 miR-124기인rs531564기인다태성여엄동지구한족부녀궁경암적유전역감유밀절관계，G등위기인가능시강저궁경암발병풍험적중요인소。
Objective To investigate the correlation between miR-124 rs531564 polymorphisms and the susceptibility to cervical cancer in Chinese Han women in Guangdong Province. Methods The genotypes of miR-124 rs531564 polymorphism were determined using polymerase chain reaction-based ligase detection reaction (PCR-LDR) in 107 cervical cancer patients and 208 healthy female blood donors. The correlation between the polymorphism and the susceptibility to cervical cancer was evaluated using unconditional logistic regression analysis. Results The incidence of HPV infection in the patients (93.1%) was much higher than that in the control subjects (16.8%, P<0.001), suggesting the importance of HPV infection as a critical risk factor for cervical cancer. The G allele of miR-124 rs531564 polymorphism in the cervical cancer patients was much less frequent than that in the controls (8.0%vs 15.1%, P=0.014), suggesting its possible role as a protective allele. Compared with those carrying CC genotype, individuals carrying the CG and GG genotypes showed a significantly reduced risk for cervical cancer (OR=0.47, 95%CI=0.26-0.88, P=0.017), and this protective role of the G allele was more prominent in older women (≥45 years old) (OR=0.28, 95% CI=0.10-0.76, P=0.012). Conclusion miR-124 rs531564 polymorphism may play a role in cervical cancer susceptibility in Chinese Han women, and G allele is associated with a reduced risk of cervical cancer.
