CAJ | 학술논문

目的：探讨miR-520a对ErbB4的调控及其对食管鳞癌生物学行为的影响。方法应用分子生物学技术构建miR-520a的真核表达质粒、ErbB4的野生型及突变性3'-UTR报告基因，报告基因分析miR-520a对ErbB4的调控，蛋白印迹检测转染miR-520a对ErbB4蛋白表达水平的影响。应用MTT、Transwell侵袭试验探讨miR-520a对食管鳞癌细胞株增殖、凋亡及侵袭等生物学行为的影响。结果报告基因分析显示miR-520a能够下调ErbB4的野生型3'-UTR报告基因的荧光表达，而对突变性报告基因的荧光表达影响不大。蛋白印迹显示miR-520a可以显著抑制ErbB4的蛋白表达水平。此外，miR-520a可以显著抑制细胞增殖并抑制侵袭。结论 miR-520a调控ErbB4的表达并能够抑制食管鳞癌细胞的增殖与侵袭，miR-520a在食管鳞癌中发挥抑癌基因功能。
목적：탐토miR-520a대ErbB4적조공급기대식관린암생물학행위적영향。방법응용분자생물학기술구건miR-520a적진핵표체질립、ErbB4적야생형급돌변성3'-UTR보고기인，보고기인분석miR-520a대ErbB4적조공，단백인적검측전염miR-520a대ErbB4단백표체수평적영향。응용MTT、Transwell침습시험탐토miR-520a대식관린암세포주증식、조망급침습등생물학행위적영향。결과보고기인분석현시miR-520a능구하조ErbB4적야생형3'-UTR보고기인적형광표체，이대돌변성보고기인적형광표체영향불대。단백인적현시miR-520a가이현저억제ErbB4적단백표체수평。차외，miR-520a가이현저억제세포증식병억제침습。결론 miR-520a조공ErbB4적표체병능구억제식관린암세포적증식여침습，miR-520a재식관린암중발휘억암기인공능。
Objective To investigate the role of miR-520a in regulation ErbB4 expression and the biological behavior of esophageal squamous cell carcinoma (ESCC). Methods The role of miR-520a in regulating the expression of ErbB4 was investigated by Western blotting and luciferase reporter assay system. The effect of miR-520a on the proliferation and invasion of ESCC cells was detected by MTT and Transwell invasion assay, respectively. Results Western blotting and luciferase reporter assay revealed that miR-520a down-regulated the expression of ErbB4 in vitro. miR-520a significantly inhibited the proliferation and suppressed the invasion of ESCC cell line Eca109. Conclusion miR-520a regulates the expression of ErbB4 and suppresses the proliferation and invasion of ESCC cells in vitro, suggesting its role as a tumor suppressor.