CAJ | 학술논문

检测荷斯坦奶牛亚临床酮病血清中的生化指标，研究新疆亚临床酮病奶牛血液中生化指标的特点。选择产后1 d～15 d的荷斯坦亚临床酮病奶牛，通过应用自动生化分析仪、紫外分光光度计和酶联免疫分析法测定血清中BHBA、NEFA、GLU、BUN、TP、ALB、AST、ALT、TBI、TG、CT含量。结果表明，血清中GLU、ALB、CT的含量极显著低于对照组水平（P＜0．01）；血清中 BHBA、BUN、NEFA、TG、AST 和ALT含量极显著高于对照组水平（P＜0．01）；血清中 TP显著低于对照组水平（P＜0．05）。血清中 TBI显著高于对照组水平（P＜0．05）。由此提示，血清中各生化指标的含量均高于报道水平，可将血清中 BHBA的含量1．40 mmol/L作为诊断亚临床酮病的标准。血清中 GLU 、TG、NEFA等的含量可作为诊断亚临床酮病的辅助参考指标。
검측하사탄내우아림상동병혈청중적생화지표，연구신강아림상동병내우혈액중생화지표적특점。선택산후1 d～15 d적하사탄아림상동병내우，통과응용자동생화분석의、자외분광광도계화매련면역분석법측정혈청중BHBA、NEFA、GLU、BUN、TP、ALB、AST、ALT、TBI、TG、CT함량。결과표명，혈청중GLU、ALB、CT적함량겁현저저우대조조수평（P＜0．01）；혈청중 BHBA、BUN、NEFA、TG、AST 화ALT함량겁현저고우대조조수평（P＜0．01）；혈청중 TP현저저우대조조수평（P＜0．05）。혈청중 TBI현저고우대조조수평（P＜0．05）。유차제시，혈청중각생화지표적함량균고우보도수평，가장혈청중 BHBA적함량1．40 mmol/L작위진단아림상동병적표준。혈청중 GLU 、TG、NEFA등적함량가작위진단아림상동병적보조삼고지표。
Through detecting the biochemical indexes in the serum of Holstein cows with subclinical keto-sis,we studyed the characteristics of biochemical indexes in the serum of Holstein cows with subclinical ke-tosis in Xin Jiang.The 1-15 d postpartum Holstein cows with subclinical ketosis were used.Through the application of automatic biochemical analyzer,UV spectrophotometer and enzyme-linked immunosorbent to assay the contents of BHBA,NEFA,GLU,BUN,TP,ALB,AST,ALT,TBI,TG,CT in serum.Test re-sults showed that the contents of GLU,ALB,CT in serum were significantly lower than that in the control group(P<0.01);the contents of BHBA,BUN,NEFA,TG,AST and ALT were significantly higher than that in the control group(P<0.01);the content of TP was lower than that in the control group(P<0.05);the content of TBI higher than the control group level(P<0.05).These results indicated that the serum biochemical indexes were higher than that in reported levels,thus,serum BHBA can be content 1 .40 mmol/L as the diagnostic criterion of subclinical ketosis.And serum GLU,TG,NEFA and other contents can be used as diagnostic aids for subclinical ketosis.