动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
3期
125-127
,共3页
徐春志%方英%蔺俐仲%白璧%袁翠霞%李永明
徐春誌%方英%藺俐仲%白璧%袁翠霞%李永明
서춘지%방영%린리중%백벽%원취하%리영명
布鲁菌病%血清学诊断方法%符合率%比较
佈魯菌病%血清學診斷方法%符閤率%比較
포로균병%혈청학진단방법%부합솔%비교
Brucellosis%serological diagnostic methods%conformity rate%comparison
为给奶牛布鲁菌病诊断提供技术参考,采用虎红平板凝集试验(RBT)、试管凝集试验(SAT)和补体结合试验(CFT)三种血清学方法分别对420份奶牛血清样本进行比较检测。结果表明,RBT、SAT和CFT的阳性检出率依次为13.81%(58/420)、9.29%(39/420)和8.81%(37/420)。以 SAT定量检测结果为参照标准,CFT的符合率较RBT高,且待检血清CFT溶血程度与SAT抗体效价呈现一定的相关性。
為給奶牛佈魯菌病診斷提供技術參攷,採用虎紅平闆凝集試驗(RBT)、試管凝集試驗(SAT)和補體結閤試驗(CFT)三種血清學方法分彆對420份奶牛血清樣本進行比較檢測。結果錶明,RBT、SAT和CFT的暘性檢齣率依次為13.81%(58/420)、9.29%(39/420)和8.81%(37/420)。以 SAT定量檢測結果為參照標準,CFT的符閤率較RBT高,且待檢血清CFT溶血程度與SAT抗體效價呈現一定的相關性。
위급내우포로균병진단제공기술삼고,채용호홍평판응집시험(RBT)、시관응집시험(SAT)화보체결합시험(CFT)삼충혈청학방법분별대420빈내우혈청양본진행비교검측。결과표명,RBT、SAT화CFT적양성검출솔의차위13.81%(58/420)、9.29%(39/420)화8.81%(37/420)。이 SAT정량검측결과위삼조표준,CFT적부합솔교RBT고,차대검혈청CFT용혈정도여SAT항체효개정현일정적상관성。
In order to provide technical reference for diagnosis of brucellosis in dairy cattle,420 serum sam-ples were detected and compared by RBT,SAT and CFT.The results showed that the positive rates of RBT,SAT and CFT were 13.81%,9.29% and 8.81%.Compared with the results of quantitative detection by SAT,the conformity rate of CFT was higher than that of RBT,and the corelation was observed between CFT hemolytic degree and SAT antibody titer.