CAJ | 학술논문

西尼罗病毒病（WND）是由西尼罗病毒（WNV）感染引起的一种人兽共患的急性传染病，应用各种诊断技术对该病进行准确、及时的诊断是预防和控制该病的前提。论文就近年来 WND诊断技术研究进展情况进行综述，包括病原学诊断、血清学诊断和分子生物学诊断。在病原学诊断方面，主要包括病毒分离鉴定、抗原捕获ELISA及基于膜的电化学生物传感器技术等。在血清学诊断方面，主要包括 ELISA方法、补体依赖性细胞毒性反应、基于微球的免疫测定方法及表面增强拉曼散射等。在分子生物学诊断方面，主要包括RT-PCR和RT-nPCR、real-time RT-PCR、实时快速反转录环介导恒温扩增及依赖核酸序列的扩增技术等，并对各种方法的前景和优缺点进行了阐述，为实际应用及新方法的建立提供参考。
서니라병독병（WND）시유서니라병독（WNV）감염인기적일충인수공환적급성전염병，응용각충진단기술대해병진행준학、급시적진단시예방화공제해병적전제。논문취근년래 WND진단기술연구진전정황진행종술，포괄병원학진단、혈청학진단화분자생물학진단。재병원학진단방면，주요포괄병독분리감정、항원포획ELISA급기우막적전화학생물전감기기술등。재혈청학진단방면，주요포괄 ELISA방법、보체의뢰성세포독성반응、기우미구적면역측정방법급표면증강랍만산사등。재분자생물학진단방면，주요포괄RT-PCR화RT-nPCR、real-time RT-PCR、실시쾌속반전록배개도항온확증급의뢰핵산서렬적확증기술등，병대각충방법적전경화우결점진행료천술，위실제응용급신방법적건립제공삼고。
West Nile virus disease (WND)is a seriously infectious zoonosis caused by West Nile virus (WNV).The application of different diagnostic tests for accurate and timely diagnosis of the disease is the premise for the prevention and control of WND.This article mainly reviewed the latest research progress on diagnostic techniques for WND.The diagnostic methods of WND include pathogenical,serological and molecular diagnoses.In the pathogenical detection aspect,these techniques include virus isolation and iden-tification,antigen capture ELISA and membrane-based electrochemical nanobiosensor.In the serological detection aspect,mainly include ELISA,complement-dependent cytotoxicity (CDC),microsphere-based im-munoassay (MIA)and surface enhanced Raman scattering immunoassay(SERS).In molecular detection aspect,mainly include RT-PCR,RT-nPCR,real-time RT-PCR,loop-mediated isothermal amplification as-say (LAMP)and nucleic acid sequence-based amplification assay (NASBA).In addition,the advantages, disadvantages and prospects of the several methods were also discussed,to provide some references for ap-plication and future research.