CAJ | 학술논문

为了探讨热应激条件下内毒素对肉鸡肾脏的损伤作用，复制肉鸡热应激模型。采用鲎试剂法和生物化学方法检测不同热应激持续时间血清中内毒素、肌酐、尿素氮含量的变化；采用分子生物学方法检测肾脏内毒素特异性受体TLR4 mRNA表达的影响。结果显示，与对照组相比，热应激组肉鸡血清中内毒素、肌酐和尿素氮含量均极显著升高，肾脏中TLR4 mRNA水平显著上调。表明热应激刺激能使肉鸡血清中内毒素含量升高并对肉鸡肾脏造成损伤，肾脏的损伤程度与热应激持续时间呈正相关。
위료탐토열응격조건하내독소대육계신장적손상작용，복제육계열응격모형。채용후시제법화생물화학방법검측불동열응격지속시간혈청중내독소、기항、뇨소담함량적변화；채용분자생물학방법검측신장내독소특이성수체TLR4 mRNA표체적영향。결과현시，여대조조상비，열응격조육계혈청중내독소、기항화뇨소담함량균겁현저승고，신장중TLR4 mRNA수평현저상조。표명열응격자격능사육계혈청중내독소함량승고병대육계신장조성손상，신장적손상정도여열응격지속시간정정상관。
To investigate endotoxin-induced renal impairment in broilers under heat stress,we produced ani-mal model of heat stress.The variations of contents in endotoxin,creatinine and urea nitrogen in serum were detected by Limulus reagent test and biochemical methods during heat stress.The endotoxin-specific receptor TLR4 in kidney was detected by molecular biology methods during heat stress.The results showed that,compared with the control group,the contents of endotoxin,creatinine and urea nitrogen in serum were significantly elevated in the experimental group.The activity of TLR4 mRNA significantly ele-vated in the experimental group.It indicated that heat stress may cause higher purity of endotoxin and re-nal impairment in broilers.The degree of renal impairment and the duration of heat stress was positively correlated.