CAJ | 학술논문

近年来，鸡传染性支气管炎（IB）在我国广西地区相继暴发，流行十分广泛，给养禽业带来巨大损失。为及时了解传染性支气管炎病毒（IBV）的变异情况，从而进行更有效的防控，本研究对2012年分离自广西的8株IBV的S1基因进行了序列测定与分析。参考NCBI上的IBV S1基因序列，设计合成了一对扩增S1基因全长序列的引物。采用 RT-PCR方法对8株 IBV分离株（GX1-GX8）的 S1基因进行克隆测序。序列分析结果表明，8株IBV分离株S1基因全长均为1620 bp，且集中分布在2个分支，GX1-GX4属于第Ⅴ分支，其裂解位点均为RRFRR；GX5-GX8属于第Ⅰ分支，其裂解位点均为 HRRRR，为 IBV中国毒株所特有。同时，这些分离株进化趋势与其分离时间之间显示出一定的相关性；潜在糖基化位点（PGS）分析结果显示，GX1-GX4的PGS分布一致且与GX5-GX8的分布显示出一定的差异；核苷酸同源性分析结果表明，位于同一分支的毒株间同源性较高，所有分离毒株与我国使用的常规 Mass 型疫苗毒株的同源性均偏低。这暗示目前广西IBV分离毒株与我国广泛使用的IB疫苗毒株的遗传进化距离较远，常规Mass型疫苗可能起不到很好的免疫保护作用。
근년래，계전염성지기관염（IB）재아국엄서지구상계폭발，류행십분엄범，급양금업대래거대손실。위급시료해전염성지기관염병독（IBV）적변이정황，종이진행경유효적방공，본연구대2012년분리자엄서적8주IBV적S1기인진행료서렬측정여분석。삼고NCBI상적IBV S1기인서렬，설계합성료일대확증S1기인전장서렬적인물。채용 RT-PCR방법대8주 IBV분리주（GX1-GX8）적 S1기인진행극륭측서。서렬분석결과표명，8주IBV분리주S1기인전장균위1620 bp，차집중분포재2개분지，GX1-GX4속우제Ⅴ분지，기렬해위점균위RRFRR；GX5-GX8속우제Ⅰ분지，기렬해위점균위 HRRRR，위 IBV중국독주소특유。동시，저사분리주진화추세여기분리시간지간현시출일정적상관성；잠재당기화위점（PGS）분석결과현시，GX1-GX4적PGS분포일치차여GX5-GX8적분포현시출일정적차이；핵감산동원성분석결과표명，위우동일분지적독주간동원성교고，소유분리독주여아국사용적상규 Mass 형역묘독주적동원성균편저。저암시목전엄서IBV분리독주여아국엄범사용적IB역묘독주적유전진화거리교원，상규Mass형역묘가능기불도흔호적면역보호작용。
Infectious bronchitis virus has caused huge economic losses to poultry industry in the domestic re-cently because of successive and extensive outbreak.In order to master the variation of IBV timely,this study carried out the sequence analysis of isolates from Guangxi.The S1 genes of 8 IBV isolates which were isolated from Guang Xi province of China in 2012 were cloned and sequenced by reverse transcription polymerase chain reaction (RT-PCR).Sequence analysis showed that the total length of S1 genes of 8 iso-lates was 1 620 bp,and they were classified into 2 branches.GX1-GX4 belongs to V branch,and the cleav-age motifs of them were RRFRR sequences,while GX5-GX8 belongs to branchⅠ,and their cleavage motifs were HRRRR sequences which exhibits characteristics of local pandemic strain in China.The Potential gly-cosylation site (PGS)analysis indicated that GX1-GX4 owned the same PGS distribution while differed from GX5-GX8.Both the homology alignment and phylogenetic classification with reference strains demon-strated that the 8 isolates were evolutionarily distant from all commercial vaccines.