CAJ | 학술논문

为了探究体外培养小鼠乳腺上皮细胞的消化方法，体外分离小鼠乳腺组织，分别采用胶原酶和胰蛋白酶混合消化法或两步消化法消化小鼠乳腺组织，并进行细胞培养，利用角蛋白18激光共聚焦显微技术鉴定目的细胞。结果显示，混合酶消化法能在短期内分离到大量的乳腺上皮细胞，且细胞纯度达到98％，细胞可连续培养至第3代；两步酶消化法虽能分离到一定量的乳腺上皮细胞，但细胞继代培养困难，且细胞不易纯化。以上结果表明，混合酶消化法培养小鼠乳腺上皮细胞优于两步酶消化法。
위료탐구체외배양소서유선상피세포적소화방법，체외분리소서유선조직，분별채용효원매화이단백매혼합소화법혹량보소화법소화소서유선조직，병진행세포배양，이용각단백18격광공취초현미기술감정목적세포。결과현시，혼합매소화법능재단기내분리도대량적유선상피세포，차세포순도체도98％，세포가련속배양지제3대；량보매소화법수능분리도일정량적유선상피세포，단세포계대배양곤난，차세포불역순화。이상결과표명，혼합매소화법배양소서유선상피세포우우량보매소화법。
In order to explore the enzyme digestion on mouse mammary epithelial cells(MECs)cultureed in vitro,the mammary tissue was isolated from mice aseptically in vitro,collagenase/trypsin digestion and two-step enzyme digestion were used to culture mouse MECs.The keratin 18,a specific marker for epithe-lial cell,was identifiedby confocal lasers scanning microscope.The results showed that plenty of pure mouse MECs can be harvested by using collagenase/trypsin digestion and continuously cultured for 3 generations. A certain amount of cells can be harvested with two-step enzyme digestion,but cells were subcultured dif-ficultly,and hard to purify.These results suggest that collagenase/trypsin digestion can be preferably used to culture mouse MECs.