CAJ | 학술논문

2012年我国部分省市规模化猪场副猪嗜血杆菌分离鉴定及菌株血清分型
2012년아국부분성시규모화저장부저기혈간균분리감정급균주혈청분형
Isolation,Identification and Serotyping of Haemophilus parasuis in Parts of Larg-scale Pig Farms in China 2 0 1 2

万方数据

动物医学进展動物醫學進展 동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年 3期 48-52 ,共5页

王建%邵卫星%吕占军%王超%董雅琴%刘爽%王博扬%吴发兴%张志%刘自立%王佳%李晓成

왕건%소위성%려점군%왕초%동아금%류상%왕박양%오발흥%장지%류자립%왕가%리효성

副猪嗜血杆菌%分离%鉴定%聚合酶链反应%血清分型副豬嗜血桿菌%分離%鑒定%聚閤酶鏈反應%血清分型
부저기혈간균%분리%감정%취합매련반응%혈청분형
Haemophilus parasuis%isolation%identification%PCR%serotyping

为掌握2012年我国猪场副猪嗜血杆菌（Hps）感染的发病状况，在我国部分省市规模化猪场采集疑似副猪嗜血杆菌病猪的心包积液及发生纤维素性浆膜炎的肺脏等病料共168份，通过病原分离培养、形态及培养特性观察、生化试验、PCR 扩增等方法进行鉴定，最终分离出51株副猪嗜血杆菌，分离率约为30．4％。对51株副猪嗜血杆菌用琼脂扩散法进行血清分型，得到4型的6株（11．8％），5型的9株（17．6％），13型9株（17．6％），12型、14型各2株，1型、6型、10型、11型各1株，未能确定血清型19株，4型、5型、13型占总比例的47．1％，占有毒力血清型（强毒和中等毒力血清型）比例80．0％，表明副猪嗜血杆菌4型、5型、13型菌株是我国的优势血清型。
위장악2012년아국저장부저기혈간균（Hps）감염적발병상황，재아국부분성시규모화저장채집의사부저기혈간균병저적심포적액급발생섬유소성장막염적폐장등병료공168빈，통과병원분리배양、형태급배양특성관찰、생화시험、PCR 확증등방법진행감정，최종분리출51주부저기혈간균，분리솔약위30．4％。대51주부저기혈간균용경지확산법진행혈청분형，득도4형적6주（11．8％），5형적9주（17．6％），13형9주（17．6％），12형、14형각2주，1형、6형、10형、11형각1주，미능학정혈청형19주，4형、5형、13형점총비례적47．1％，점유독력혈청형（강독화중등독력혈청형）비례80．0％，표명부저기혈간균4형、5형、13형균주시아국적우세혈청형。
In this study,168 field samples suspected with the infection of Haemophilusparasuis (Hps)were collected from several domestic large-scale pig farms in China.Among them,5 1 samples were identified as Hps by bacterial morphological analysis,biochemistry tests and PCR.The isolation rate was 30.4%.The Hps-positive isolates were subsequently serotyped by agarose diffusion test (ADT).Thirty two isolates could be successfully serotyped,belonging to serotype 4 (6 isolates),serotype 5 (9 isolates),serotype 1 3 (9 isolates),serotype 12 (2 isolates),serotype 14 (2 isolates),serotype 1 (1 isolate),serotype 6 (1 isolate), serotype 10 (1 isolate),serotype 11 (1 isolate).Nineteen isolates could not be determined in serotyping. Collectively,24 isolates belonged to serotype 4,5 and 13,which was as high as 47.1% of the total isolates and up to 80% of the virulent isolates.These results suggest that Hps was one of the most common patho-gens in pigs in China,and seroypes 4,5 and 13 of Hps were the most prevalent ones.