CAJ | 학술논문

为了解大雁、天鹅源沙门菌相关毒力基因的分布情况，采用 PCR方法对实验室保存的45株大雁、天鹅源沙门菌的毒力岛SPI-1、SPI-2、SPI-3、SPI-4、SPI-5的毒力基因 invA、spiC和 ttrC、mgtC、SPI-4R、sopB进行检测，并随机选择3株不同血清型的相关毒力基因阳性菌株进行克隆测序分析。结果显示，45个分离株中invA、sopB基因阳性检出率为91％（41/45），spiC为89％（40/45），ttrC为84％（38/45），mgtC、SPI-4R为82％（37/45）。各毒力基因在大部分菌株中均存在，测序结果表明菌株各毒力基因的同源性均在91％以上。研究结果丰富了沙门菌毒力基因分布频率的流行病学资料。
위료해대안、천아원사문균상관독력기인적분포정황，채용 PCR방법대실험실보존적45주대안、천아원사문균적독력도SPI-1、SPI-2、SPI-3、SPI-4、SPI-5적독력기인 invA、spiC화 ttrC、mgtC、SPI-4R、sopB진행검측，병수궤선택3주불동혈청형적상관독력기인양성균주진행극륭측서분석。결과현시，45개분리주중invA、sopB기인양성검출솔위91％（41/45），spiC위89％（40/45），ttrC위84％（38/45），mgtC、SPI-4R위82％（37/45）。각독력기인재대부분균주중균존재，측서결과표명균주각독력기인적동원성균재91％이상。연구결과봉부료사문균독력기인분포빈솔적류행병학자료。
To investigate the distribution features of Salmonella virulence genes from Cygnus and Anser,45 Salmonella isolates from Cygnus and Anser were preserved in our laboratory,and the virulence genes were detected by PCR for SPI-1 invA,SPI-2 spiC and ttrC,SPI-3 mgtC,SPI-4 SPI-4R,SPI-5 sopB.In addition, the virulence genes of 3 isolates,which were isolated from different serotypes were cloned and sequenced. The results showed that genes such as invA,sopB positive detection rate was 91% (41/45),spiC was 89% (40/45),ttrC was 84% (38/45),mgtC,SPI-4R was 82% (37/45).The virulence genes exist in most of the strains,sequencing strains were homologous above 9 1% compared to the reference strains in GenBank.The results enriched epidemiological data of the Salmonella virulence gene frequency distribu-tion.